Viral agents causing lower respiratory tract infections in hospitalized children: evaluation of the Speed-Oligo® RSV assay for the detection of respiratory syncytial virus

Autor: W, Sánchez-Yebra, J A, Ávila-Carrillo, F, Giménez-Sánchez, A, Reyes-Bertos, M, Sánchez-Forte, M, Morales-Torres, A, Rojas, J, Mendoza, W, Sánchez-Yebra Romera
Rok vydání: 2011
Předmět:
Microbiology (medical)
medicine.medical_specialty
Adolescent
viruses
Diagnostic Techniques
Respiratory System
Respiratory Syncytial Virus Infections
Biology
Polymerase Chain Reaction
Sensitivity and Specificity
Article
Virus
Viral Culture
law.invention
Medical microbiology
Human metapneumovirus
Predictive Value of Tests
law
Lower Respiratory Tract Infection
parasitic diseases
medicine
Bronchiolitis
Viral
Humans
Respiratory system
Child
Polymerase chain reaction
Respiratory Syncytial Virus Infection
Respiratory tract infections
Reverse Transcriptase Polymerase Chain Reaction
Infant
Newborn
Infant
virus diseases
General Medicine
medicine.disease
biology.organism_classification
Virology
Hospitalization
Infectious Diseases
Amplification Internal Control
Bronchiolitis
Child
Preschool
Respiratory Syncytial Virus
Human
Predictive value of tests
RNA
Viral
Respiratory Syncytial Virus
Child
Hospitalized
Zdroj: European Journal of Clinical Microbiology & Infectious Diseases
ISSN: 1435-4373
0934-9723
DOI: 10.1007/s10096-011-1300-4
Popis: Respiratory syncytial virus (RSV) is the viral agent which is more frequently involved in lower respiratory tract infections (LRTIs) in infants under 1 year of age in developed countries. A new oligochromatographic assay, Speed-Oligo® RSV, was designed and optimized for the specific detection and identification of RSV subtypes A and B. The test was evaluated in 289 clinical samples from 169 hospitalized children using an immunochromatography (IC) test, virus isolation by culture, and an in-house real-time polymerase chain reaction (RT-PCR). Other viruses causing LRTIs were investigated by cell culture or PCR-based tests. Sixty-two patients were infected by RSV (36.7%). In addition, adenovirus, influenza B, parainfluenza 2, and human metapneumovirus were detected in rates ranging from 5 to 8%. A proportion of 10.1% of the patients had mixed infections. The sensitivity, specificity, and positive and negative predictive values were, respectively, 94.9, 99.4, 98.9, and 97.4% for Speed-Oligo® RSV, 92.9, 96.3, 92.9, and 96.3% for RT-PCR/RSV, and 58.4, 98.1, 93.3, and 82.6% for IC. Our rates of viral detection and co-infection were similar to those of previously reported series. Finally, we find that Speed-Oligo® RSV is a rapid and easy-to-perform technique for the detection of RSV and the identification of subtypes A and B.
Databáze: OpenAIRE
Externí odkaz: