CD56+ immune cell infiltration and MICA are decreased in breast lobules with fibrocystic changes
| Autor: | Stacey J. Winham, Rushin D. Brahmbhatt, Derek C. Radisky, Lori A. Denison, Amy C. Degnim, Jodi M. Carter, Linda M. Murphy, Muhammad Arshad, Keith L. Knutson, Tanya L. Hoskin, Marlene H. Frost, Alvaro Pena, Melody Stallings-Mann, Daniel Kerekes, Daniel W. Visscher |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Adult
Male 0301 basic medicine Cancer Research medicine.medical_specialty Pathology Natural killer cell Breast Neoplasms Benign breast disease medicine.disease_cause 03 medical and health sciences Preclinical Study 0302 clinical medicine Breast cancer Neoplasms Internal medicine medicine Humans Cytotoxic T cell Breast skin and connective tissue diseases Aged Hyperplasia biology Activating ligand MICA business.industry Histocompatibility Antigens Class I Cancer Middle Aged medicine.disease CD56 Antigen Staining Killer Cells Natural stomatognathic diseases 030104 developmental biology Endocrinology medicine.anatomical_structure Oncology 030220 oncology & carcinogenesis biology.protein Female CD56 Breast disease Antibody business Carcinogenesis Precancerous Conditions |
| Zdroj: | Breast Cancer Research and Treatment |
| ISSN: | 1573-7217 0167-6806 |
| Popis: | Purpose While the role of natural killer (NK) cells in breast cancer therapy has been investigated, little information is known about NK cell function and presence in nonmalignant and premalignant breast tissue. Here, we investigate and quantify NK cell marker CD56 and activating ligand MICA in breast tissue with benign breast disease. Methods Serial tissue sections from 88 subjects, 44 with benign breast disease (BBD) who remained cancer-free, and 44 with BBD who later developed cancer, were stained with H&E, anti-MICA, and anti-CD56. Up to ten representative lobules were identified on each section. Using digital image analysis, MICA and CD56 densities were determined for each lobule, reported as percent of pixels in the lobule that registered as stained by each antibody. Analyses were performed on a per-subject and per-lobule basis. Results Per-subject multivariate analyses showed associations of CD56 and MICA with age: CD56 was increased in older subjects (p = 0.03), while MICA was increased in younger subjects (p = 0.005). Per-lobule analyses showed that CD56 and MICA levels were both decreased in lobules with fibrocystic change, with median levels of CD56 and MICA staining, respectively, at 0.31 and 7.0% in fibrocystic lobules compared to 0.76 and 12.2% in lobules without fibrocystic change (p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink