Potential for alternative intron–exon pairings in group II intron RmInt1 from Sinorhizobium meliloti and its relatives
| Autor: | Nicolás Toro, François Michel, Maria do Carmo Costa |
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| Přispěvatelé: | Centre de génétique moléculaire (CGM), Centre National de la Recherche Scientifique (CNRS), Grupo de Ecologıa Genética, Estacion, Granada, Consejo Superior de Investigaciones Científicas [Madrid] (CSIC) |
| Rok vydání: | 2006 |
| Předmět: |
Models
Molecular MESH: Introns Sequence analysis Molecular Sequence Data MESH: Base Sequence 03 medical and health sciences Exon MESH: RNA Catalytic [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology RNA Catalytic Letter to the Editor Molecular Biology 030304 developmental biology Genetics 0303 health sciences Sinorhizobium meliloti MESH: Molecular Sequence Data Binding Sites Splice site mutation Base Sequence biology 030306 microbiology MESH: Alternative Splicing Alternative splicing Ribozyme Intron Exons Group II intron biology.organism_classification Molecular biology Introns [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biomolecules [q-bio.BM] Alternative Splicing RNA Bacterial MESH: Nucleic Acid Conformation MESH: Binding Sites biology.protein Nucleic Acid Conformation MESH: Exons MESH: RNA Bacterial MESH: Sinorhizobium meliloti MESH: Models Molecular |
| Zdroj: | RNA RNA, Cold Spring Harbor Laboratory Press, 2006, 12 (3), pp.338-41. ⟨10.1261/rna.2240906⟩ |
| ISSN: | 1469-9001 1355-8382 |
| DOI: | 10.1261/rna.2240906 |
| Popis: | Ribozyme constructs derived from group II intron RmInt1 of Sinorhizobium meliloti self-splice in vitro when incubated under permissive conditions, but exon ligation is unusually inefficient when the 5′ exon is truncated close to the IBS2 intron-binding site. One plausible explanation for this observation is the presence of an alternative intron–exon pairing between an intron segment that overlaps with the EBS2 exon-binding site and a 5′ exon site located just distal of IBS2 relative to the splice junction. Strikingly, the existence of this pairing is supported by comparative sequence analysis of introns related to RmInt1. |
| Databáze: | OpenAIRE |
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