Detection of a novel genotype of Cryptosporidium in Antarctic pinnipeds
| Autor: | Francisco Javier García-Peña, Mercedes Gómez-Bautista, Susana Pedraza-Díaz, Luis Miguel Ortega-Mora, Claudia Rengifo-Herrera, Daniel García-Párraga |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Giardiasis
Leptonychotes weddellii Seals Earless Molecular Sequence Data Protozoan Proteins Antarctic Regions Cryptosporidiosis Cryptosporidium Zoology Feces parasitic diseases Genotype RNA Ribosomal 18S Animals HSP70 Heat-Shock Proteins 18s rdna Shetland General Veterinary biology Ecology Giardia General Medicine Ribosomal RNA biology.organism_classification Caniformia Southern elephant seal Cytoskeletal Proteins Parasitology |
| Zdroj: | Veterinary Parasitology. 191:112-118 |
| ISSN: | 0304-4017 |
| DOI: | 10.1016/j.vetpar.2012.08.021 |
| Popis: | A study was conducted to investigate the presence of Cryptosporidium and Giardia in Antarctic marine mammals. A total of 270 faecal samples from different species of pinnipeds from different locations in the South Shetland Islands and Antarctic Peninsula were analysed by immunofluorescence microscopy and PCR. Cryptosporidium was detected by PCR in three samples from Southern elephant seals (Mirounga leonina) and 2 Weddell seals (Leptonychotes weddellii). However, no oocysts were observed in any of the samples by immunofluorescence microscopy. Molecular characterisation of the isolates, using the 18S rDNA, the HSP70 and the COWP loci, revealed the presence of a Cryptosporidium sp., previously reported from an Antarctic Southern elephant seal, in the elephant seals and a novel genotype in Weddell seals. Giardia could not be detected in any of the samples analysed. |
| Databáze: | OpenAIRE |
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