Hyper(ADP-ribosyl)ation of histone H1
| Autor: | Y. Brousseau, J. Miclette, V. T. Dam, Guy G. Poirier, R. J. Aubin, A. Huletsky |
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| Rok vydání: | 1982 |
| Předmět: |
Male
Poly Adenosine Diphosphate Ribose biology Rats Inbred Strains General Medicine NAD Chromatin Nucleosomes Rats Histones Histone NAD+ Nucleosidase Histone H1 Biochemistry Histone H2A Histone methylation biology.protein Nucleosome Animals Histone octamer Poly(ADP-ribose) Polymerases Pancreas Micrococcal nuclease |
| Zdroj: | Canadian journal of biochemistry. 60(12) |
| ISSN: | 0008-4018 |
| Popis: | Nucleosomal chains of various repeat unit lengths were generated by a mild micrococcal nuclease digestion of purified pancreatic nuclei. Maximal nucleosome associated poly(ADP-ribose) polymerase activity was recovered in trimeric to tetrameric chromatin fragments, after which the enzyme activity gradually decreased and stabilized towards oligomeric periodicities of 11 to 16 nucleosomes. Electrophoresis of [32P] ADP-ribosylated histones on first-dimension acid–urea or acid–urea–Triton gels and on second-dimension acid – urea – cetyltriammonium bromide gels revealed that, of all histones, only histone H1 could be significantly poly(ADP-ribosyl)ated while only minimal modification could be recovered with histone H10. Furthermore, the extent of ADP-ribosylation present on pancreatic histone H1 is shown to proportionally retard this protein's electrophoretic mobility in all gel systems and to consist of a distinct series of at least 12 modification intermediates which can be evidenced, in nuclei or nucleosomes, and fully recovered along with histone H1 upon its selective extraction with 5% perchloric acid. The generation of these increasingly ADP-ribosylated forms of histone H1 is also demonstrated to be time dependent and the more complex ADP-ribosylated forms of this histone are favored at high NAD+ concentrations. Moreover, the electrophoretic mobilities of all intermediates are unaffected by the presence of the nonionic detergent Triton X-100. |
| Databáze: | OpenAIRE |
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