Elemental bioimaging of manganese uptake in C. elegans
| Autor: | Rebecca Niehaus, Uwe Karst, Julia Bornhorst, Christoph A. Wehe, Sigrid Grosse Brinkhaus, Olga Reifschneider, Michael Aschner, Sudipta Chakraborty |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
ved/biology.organism_classification_rank.species
Mutant Biophysics Analytical chemistry chemistry.chemical_element Manganese Biology Biochemistry Article Mass Spectrometry Biomaterials Metal Laser therapy Animals Model organism Caenorhabditis elegans Extramural ved/biology Metals and Alloys Biological Transport biology.organism_classification chemistry Chemistry (miscellaneous) visual_art visual_art.visual_art_medium Laser Therapy |
| Popis: | A new method for elemental bioimaging with laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) was developed and applied to study the uptake of manganese (Mn) in Caenorhabditis elegans (C. elegans). C. elegans is a well-established model organism in neuroscience, genetics and genomics, which has been extensively studied to decipher mechanisms of heavy metal induced toxicity. Knowledge about the distribution of manganese (Mn) and other metals in this organism will be helpful in elucidating pathways and mechanisms of transport, distribution and excretion. The LA-ICP-MS method requires limited sample preparation and can be used rapidly and easily to visualize the Mn distribution in C. elegans. Due to thorough optimization of the analytical parameters, intense Mn signals in C. elegans wild-type (WT) and mutants were obtained at a spatial resolution as small as 4 μm, thus proving the suitability of LA-ICP-MS to study the uptake of metals in C. elegans. |
| Databáze: | OpenAIRE |
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