Evidence for antimicrobial and anticancer activity of pituitary adenylate cyclase-activating polypeptide (PACAP) from North African catfish (Clarias gariepinus) Its potential use as novel therapeutic agent in fish and humans
| Autor: | Reynold Morales, Carolina Tafalla, Brizaida Oliva, Aymé Oliva, Carolina Aquilino, Tirso Pons, Mario Pablo Estrada, Juana María Lugo |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Clarias gariepinus endocrine system Erythrocytes Lung Neoplasms Antimicrobial peptides Neuropeptide Antineoplastic Agents Aquatic Science Pharmacology PACAP medicine.disease_cause Hemolysis 03 medical and health sciences Immune system Anti-Infective Agents Carcinoma Non-Small-Cell Lung Cell Line Tumor Candida albicans In silico studies medicine Animals Humans Environmental Chemistry Receptor Catfishes Cell Proliferation Bacteria Cytotoxic activity biology Bacterial and fungal pathogens Cell-penetrating peptides Pathogenic bacteria 04 agricultural and veterinary sciences General Medicine biology.organism_classification Aeromonas hydrophila Fish 030104 developmental biology Cancer cell 040102 fisheries Pituitary Adenylate Cyclase-Activating Polypeptide 0401 agriculture forestry and fisheries Anticancer peptides hormones hormone substitutes and hormone antagonists Antimicrobial Cationic Peptides Catfish |
| Zdroj: | Repositorio de Resultados de Investigación del INIA INIA: Repositorio de Resultados de Investigación del INIA Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria INIA |
| Popis: | Pituitary adenylate cyclase-activating polypeptide (PACAP) is a regulatory neuropeptide that belongs to the secretin/glucagon superfamily, of which some members have shown antimicrobial activities. Contrasting to mammals, published studies on the action of PACAP in non-mammalian vertebrate immune system remain scarce. Some of our recent studies added this peptide to the growing list of mediators that allow cross-talk between the nervous, endocrine and immune systems in teleost fish. Regulation of PACAP and expression of its receptor genes has been demonstrated during an immune response mounted against acute bacterial infection in fish, though the direct effect of PACAP against fish pathogenic bacteria has never been addressed. Current work provides evidence of antimicrobial activity of Clarias gariepinus PACAP against a wide spectrum of Gram-negative and Gram-positive bacteria and fungi of interest for human medicine and aquaculture, in which computational prediction studies supported the putative PACAP therapeutic activity. Results also indicated that catfish PACAP not only exhibits inhibitory effects on pathogen growth, but also affects the proliferation of human non-small cell lung cancer cell line H460 in a dose-dependent manner. The observed cytotoxic activity of catfish PACAP against human tumor cells and pathogenic microorganisms, but not healthy fish and mammalian erythrocytes support a potential physiological role of this neuropeptide in selective microbial and cancer cell killing. All together, our findings extend the mechanisms by which PACAP could contribute to immune responses, and open up new avenues for future therapeutic application of this bioactive neuropeptide. |
| Databáze: | OpenAIRE |
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