Molecular genetic mechanisms of influence of laser radiation with 577 nm wavelength in a microimpulse mode on the condition of the retina
| Autor: | Sergey Anatolievich Borzenok, Natalya Alexandrovna Gavrilova, Aleksandra V. Zinov'eva, Vladislav A. Solomin, Olga Evgen'evna Tishchenko, Dmitri V. Zaletaev, Olga U. Komova, Nuriya S. Gadzhieva |
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| Rok vydání: | 2019 |
| Předmět: |
Male
Vascular Endothelial Growth Factor A 0301 basic medicine medicine.medical_treatment Neurotransmission GPI-Linked Proteins Retina law.invention 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine law medicine Animals Eye Proteins Molecular Biology Saline Uridine Phosphorylase Chemistry Membrane Proteins Membrane transport Laser Recombinant Proteins Sensory Systems Epithelium Cell biology Mice Inbred C57BL Neuroepithelial cell Ophthalmology 030104 developmental biology medicine.anatomical_structure Gene Expression Regulation Insulin-Like Growth Factor Binding Protein 4 Intravitreal Injections 030221 ophthalmology & optometry Recombinant DNA Laser Therapy sense organs |
| Zdroj: | Experimental Eye Research. 185:107650 |
| ISSN: | 0014-4835 |
| Popis: | The aim of the study was to investigate the molecular genetic mechanisms of the influence of laser radiation with 577 nm wavelength in a microimpulse mode on the retina in the experimental conditions after the intravitreal injection of VEGF. Materials and methods The study was performed on 4-5 week-old male mice of the line C57BL/6J. The animals were divided into 4 groups of 5 mice in each group, one eye was excremental, the contralateral eye remained intact. In the first group, intravitreal injection of PBS was performed; in the second group, intravitreal injection of 50 ng/ml of recombinant VEGF165 in 2 μL of phosphate-buffered saline (PBS) was performed; in the third and fourth groups, a day after the intravitreal injection of recombinant VEGF165, laser radiation with wavelength 577 nm was applied in the micropulse and continuous modes, respectively. Tissue samples (neuroepithelium, pigment epithelium) for the microarray transcription analysis in the animals from group 1 and 2 were taken 2 days after the injection of PBS and VEGF, in the animals from group 3 and 4 – a day after the retina was exposed to laser radiation. Results and conclusion Molecular genetic mechanisms of the influence of laser radiation with wavelength 577 nm in a microimpulse mode on the retina in experimental conditions were studied and the genes that significantly changed the level of expression (the genes that take part in the regulation of neoangiogenesis, structural cell functions, processes of cells proliferation, transcription, differentiation, transmembrane transport, signaling, synaptic transmission, etc.) were identified. |
| Databáze: | OpenAIRE |
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