In-vitro cell tests using doxorubicin-loaded polymeric TiO2 nanotubes used for cancer photothermotherapy
| Autor: | Soon-Sun Hong, Mikwon Son, Soyeon An, Chongmu Lee, Chanseok Hong, Don Haeng Lee |
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| Rok vydání: | 2012 |
| Předmět: |
Cancer Research
Cell Survival Surface Properties Apoptosis Mice Surface-Active Agents chemistry.chemical_compound Microscopy Electron Transmission Pulmonary surfactant Cell Line Tumor Neoplasms medicine Animals Pharmacology (medical) Doxorubicin MTT assay Irradiation Viability assay Particle Size Fluorescein isothiocyanate Titanium Pharmacology Antibiotics Antineoplastic Nanotubes Lasers Hyperthermia Induced Flow Cytometry Oncology chemistry Polyvinyl Alcohol Cancer cell Particle size medicine.drug Nuclear chemistry |
| Zdroj: | Anti-Cancer Drugs. 23:553-560 |
| ISSN: | 0959-4973 |
| DOI: | 10.1097/cad.0b013e328350446b |
| Popis: | To determine the appropriate surfactant to be added to TiO(2) nanotubes (TNTs) for use in cancer photothermotherapy, this study measured the increase in temperature and examined the size distribution of TNT particles loaded with different surfactants during near-infrared irradiation. In addition, in-vitro cell (fluorescein isothiocyanate and MTT assay) tests were carried out to examine the cytotoxic effect of doxorubicin-loaded and polyvinyl alcohol-added TNTs (pTNTs). The mean particle size of the pTNTs was 151.8 nm with a particle size variation of less than 3 nm, which is low enough to flow through blood vessels without causing a blockage. The temperature of the pTNTs was ∼47°C, which is high enough to destroy cancer cells. Doxorubicin-loaded TNTs and pTNTs in combination with a near-infrared laser showed a cell viability of 4.5% - a sufficiently high cytotoxic effect. |
| Databáze: | OpenAIRE |
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