Methanotrophic production of polyhydroxybutyrate-co-hydroxyvalerate with high hydroxyvalerate content
| Autor: | Diana Franqui-Villanueva, Maria I. Ponce, W. Dirk Sikkema, Charles C. Lee, William J. Orts, Allison J. Pieja, Timothy J. Riiff, Andrew J. Cal |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Valeric acid Methanotroph Polyesters 030106 microbiology Valerate Biochemistry Polyhydroxyalkanoates Microbiology Polyhydroxybutyrate 03 medical and health sciences chemistry.chemical_compound Crystallinity Pentanols Structural Biology Pentanoic Acids Molecular Biology chemistry.chemical_classification biology Biological Transport General Medicine biology.organism_classification 030104 developmental biology Monomer chemistry Chemical engineering Methane Methylocystaceae Bacteria Copper |
| Zdroj: | International journal of biological macromolecules. 87 |
| ISSN: | 1879-0003 |
| Popis: | Type II methanotrophic bacteria are a promising production platform for PHA biopolymers. These bacteria are known to produce pure poly-3-hydroxybutyrate homopolymer (PHB). We isolated a strain, Methylocystis sp. WRRC1, that was capable of producing a wide range of polyhydroxybutyrate-co-hydroxyvalerate copolymers (PHB-co-HV) when co-fed methane and valerate or n-pentanol. The ratio of HB to HV monomer was directly related to the concentration of valeric acid in the PHA accumulation media. We observed increased incorporation of HV and total polymer under copper-free growth conditions. The PHB-co-HV copolymers produced had decreased melting temperatures and crystallinity compared with methanotroph-produced PHB. |
| Databáze: | OpenAIRE |
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