DNA as a Recyclable Natural Polymer
Autor: | Francesco Stellacci, Weina Liu, Daniel Ortiz, Simone Giaveri |
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Rok vydání: | 2022 |
Předmět: |
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nucleoside dna recycling one-pot phosphorylation law.invention qpcr Biomaterials cell lysate chemistry.chemical_compound dna hydrolysis law Electrochemistry Nucleotide Polymerase chain reaction chemistry.chemical_classification Polymer Ribosomal RNA Condensed Matter Physics nucleotides Amino acid Electronic Optical and Magnetic Materials Real-time polymerase chain reaction chemistry Biochemistry Yield (chemistry) escherichia-coli methylation DNA |
Zdroj: | Advanced Functional Materials. 32:2109538 |
ISSN: | 1616-3028 1616-301X |
DOI: | 10.1002/adfm.202109538 |
Popis: | Nature has the ability of circularly re-using its components to produce molecules and materials it needs. An example is the ability of most living organisms of digesting proteins they feed off into amino acids and then using such amino acids in the ribosomal synthesis of new proteins. Recently, we have shown that such recycling of proteins can be reproduced outside living organisms. The key proteins’ feature that allows for this type of recycling is their beingsequence-defined polymers. Arguably, Nature’smost famous sequence-defined polymer is DNA. Here we show that it is possible starting from sheared calf-DNA to obtain all the four nucleotides as monophosphate-nucleotides (dNMPs). These dNMPs were phosphorylated in a one-pot, multi-enzymes, phosphorylation reaction to generate triphosphate-nucleotides (dNTPs). Finally, we used the dNTPs so achieved (with a global yield of ~60%) as reagents for PCR (polymerase chain reaction) to produce target DNA strands, and for the diagnose of targeted DNA by quantitative PCR (qPCR). This approach is an efficient, convenient, and environmentally friendly way to produce dNTPs and DNA through recycling according to the paradigm of circular economy. |
Databáze: | OpenAIRE |
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