Bta-miR-2400 Targets SUMO1 to Affect Yak Preadipocytes Proliferation and Differentiation
| Autor: | Lanhua Ma, Yongfang Chang, Xian Guo, Ding Xuezhi, Bao Pengjia, Yarong Gu, Chunnian Liang, Yongfeng Zhang, Min Chu, Ping Yan |
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| Rok vydání: | 2021 |
| Předmět: |
Messenger RNA
SUMO1 General Immunology and Microbiology QH301-705.5 Cell growth proliferation Cyclin-dependent kinase 2 Adipose tissue Lipid metabolism differentiation Biology Article General Biochemistry Genetics and Molecular Biology Energy homeostasis Cell biology microRNA biology.protein Proliferation Marker yak adipocyte Biology (General) General Agricultural and Biological Sciences bta-miR-2400 |
| Zdroj: | Biology, Vol 10, Iss 949, p 949 (2021) Biology Volume 10 Issue 10 |
| ISSN: | 2079-7737 |
| DOI: | 10.3390/biology10100949 |
| Popis: | Simple Summary Yak adipose tissue may have evolved a unique energy metabolism manner that accommodates the organism’s seasonal growth rhythms. Rare reports have shown that miRNAs regulate lipid metabolism in domestic yaks. In the present study, miR-2400 is a novel bovine miRNA; gain- and loss- function of bta-miR-2400 were performed in Yak preadipocytes, and it was revealed that bta-miR-2400 regulates lipid metabolism and energy homeostasis in yak preadipocytes by directly targeting small ubiquitin like modifier 1 (SUMO1) to promote cell proliferation and inhibit differentiation. These findings will undoubtedly facilitate future studies of miRNA regulation in lipid metabolism in high-altitude animals. Abstract Yak adipose tissue may have evolved a unique energy metabolism manner to accommodate the organism’s seasonal growth rhythms. MiRNAs regulate multiple biological processes including systemic metabolism and energy homeostasis through post-transcriptional regulations. Rare reports have shown that miRNAs regulate lipid metabolism in domestic yaks. Therefore, we investigated the regulatory mechanisms of bta-miR-2400 in modulating yak preadipocytes proliferation and differentiation. We found that bta-miR-2400 was highly expressed in adipose tissue. Overexpression of bta-miR-2400 in yak preadipocytes significantly enhanced cell proliferation, increased the number of EdU fluorescence-stained cells, and promoted the expression of proliferation marker genes (CDK2, CDK4 and PCNA). Besides, overexpression of bta-miR-2400 repressed the expression of adipogenesis-related marker genes, and the content of cellular triglyceride was substantially reduced. Conversely, inhibition of bta-miR-2400 showed opposite effects compared to those of bta-miR-2400 overexpression in yak preadipocytes. Further, luciferase reporter assays revealed that SUMO1 is a target gene of bta-miR-2400, with bta-miR-2400 being able to down-regulate SUMO1 mRNA and protein expression. In conclusion, bta-miR-2400 regulates lipid metabolism and energy homeostasis in yak preadipocytes by directly targeting SUMO1 to promote cell proliferation and inhibit differentiation. |
| Databáze: | OpenAIRE |
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