Preliminary evidence of age-dependent clinical signs associated with porcine circovirus 2b in experimentally infected CH3/Rockefeller mice
| Autor: | Cinthia K. Mori, Sueli Santos, Leonardo José Richtzenhain, Katarina B. Yamada, C. P Oliveira, Priscilla F. Gerber, Taís F. Cruz, Michelle de Paula Gabardo, Alessandra Marnie Martins Gomes de Castro, Roberto Maurício Carvalho Guedes, João Pessoa Araújo |
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| Přispěvatelé: | Universidade de São Paulo (USP), Veterinária, Universidade Federal de Minas Gerais (UFMG), Universidade Estadual Paulista (Unesp) |
| Rok vydání: | 2015 |
| Předmět: |
Circovirus
Rodent Diseases animal diseases Molecular Sequence Data Genome Viral Disease RATOS (PATOLOGIA) Rodents Mice Random Allocation Animals Viral load Circoviridae Infections Feces Swine virus General Veterinary biology Inoculation Age Factors virus diseases Sequence Analysis DNA biology.organism_classification PCV2 Porcine circovirus Immunology Nasal administration |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| Popis: | Made available in DSpace on 2018-12-11T16:59:31Z (GMT). No. of bitstreams: 0 Previous issue date: 2015-12-01 Mice and rats are susceptible to porcine circovirus 2b (PCV2) infection under field and experimental conditions. However, whether PCV2 induces disease in rodents remains a matter of debate. The objectives of the present study were to determine whether PCV2-induced disease in mice is age-dependent and whether intranasally inoculated animals are able to infect animals they come into contact with. Twenty-five CH3/Rockefeller mice were divided into six groups and intranasally inoculated with 25. μL of either PCV2b or PBS on days 0, 3 and 6. One group remained untreated. Two age groups were tested: 3-week-old mice and 6-week-old mice. The administration of three PCV2 intranasal inoculations at intervals of three days was able to induce infection and support virus transmission in susceptible mice, regardless of the age at inoculation. The clinical signs associated with PCV2 infection were more severe in younger mice, and PCV2-DNA load was higher in their faeces. In conclusion, PCV2 induced disease in mice. Departamento de Medicina Veterinária Preventiva e Saúde Animal Da Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo Cidade Universitária, Av. Prof. Dr. Orlando Marques de Paiva 87 Complexo Educacional Faculdades Metropolitana Unidas Veterinária, Rua Ministro Nelson Hungria, 541, Real Parque Departamento de Clínica e Cirurgia Veterinária Escola de Veterinária Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627 - Pampulha Departamento de Imunologia e Microbiologia Instituto de Biociência Universidade Estadual Paulista Julio de Mesquita Filho, Campus de Botucatu Biotechnology Inst. - IBTEC Sao Paulo State Univ. UNESP, Campus de Botucatu Departamento de Imunologia e Microbiologia Instituto de Biociência Universidade Estadual Paulista Julio de Mesquita Filho, Campus de Botucatu Biotechnology Inst. - IBTEC Sao Paulo State Univ. UNESP, Campus de Botucatu |
| Databáze: | OpenAIRE |
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