The C-terminal domain of Mad-like signal transducers is sufficient for biological activity in the Xenopus embryo and transcriptional activation
| Autor: | Danny Huylebroeck, Przemko Tylzanowski, Kristin Verschueren, Geert Meersseman, Claudia Blumenstock, Harry Kraft, Christine A. Kozak, Luc Nelles, Christof Niehrs, Gunther Wuytens, Jacques E. Remacle |
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| Rok vydání: | 1997 |
| Předmět: |
Transcriptional Activation
Embryology animal structures Transcription Genetic Molecular Sequence Data Xenopus Smad Proteins Biology Polymerase Chain Reaction DNA-binding protein Mice Structure-Activity Relationship Xenopus laevis Transactivation Morphogenesis Animals Amino Acid Sequence Transcription factor Gene Cell Nucleus C-terminus Chromosome Mapping Nuclear Proteins Embryo biology.organism_classification Molecular biology Cell biology DNA-Binding Proteins Trans-Activators Signal transduction Sequence Alignment Transcription Factors Developmental Biology |
| Zdroj: | Mechanisms of Development. 61:127-140 |
| ISSN: | 0925-4773 |
| DOI: | 10.1016/s0925-4773(96)00629-6 |
| Popis: | We report the characterization of two vertebrate homologs of Drosophila mothers against dpp (Mad) isolated from the mouse and the Xenopus embryo, named MusMLP (mad-likeprotein) and XenMLP, respectively, together with a summary of their expression patterns in the embryo. Overexpression of XenMLP causes ventralization of Xenopus embryos and we demonstrate that the C-terminal domain is necessary and sufficient to confer this biological effect. This domain also has the potential for transcriptional activation, as shown in one-hybrid assays in mammalian cells. We further demonstrate that MLPs are multidomain proteins by showing a cis-negative effect of the N-terminal domain on the transactivation by the C-terminal domain and that the proline-rich, middle domain maximizes the activity of the C-terminal domain. We also mapped the MusMLP gene to a region on mouse chromosome 13 that corresponds to a region on human chromosome 5q that contains cancer-related genes. |
| Databáze: | OpenAIRE |
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