Evolutionary relationships among Massospora spp. (Entomophthorales), obligate pathogens of cicadas
| Autor: | Jason E. Stajich, Kathie T. Hodge, Greg R. Boyce, Angie M. Macias, Matthew T. Kasson, Matthew C. Berger, Piotr Łukasik, David M. Geiser, DeAnna C. Bublitz, Claudio Veloso |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Polytomy synonymization Physiology Lineage (evolution) Biology 010603 evolutionary biology 01 natural sciences Magicicada 030308 mycology & parasitology 03 medical and health sciences Monophyly Genus Okanagana 28S ribosomal RNA Genetics Molecular Biology Ecology Evolution Behavior and Systematics 030304 developmental biology Platypedia 0303 health sciences Obligate Phylogenetic tree Entomophthoraceae Cell Biology General Medicine 15. Life on land biology.organism_classification entomopathogen Taxon Evolutionary biology Entomophthorales Diceroprocta invertebrate pathology Tettigades Zoopagomycota |
| Popis: | The fungal genusMassospora(Zoopagomycota: Entomophthorales) includes more than a dozen obligate, sexually transmissible pathogenic species that infect cicadas (Hemiptera) worldwide. At least two species are known to produce psychoactive compounds during infection, which has garnered considerable interest for this enigmatic genus. As with many Entomophthorales, the evolutionary relationships and host associations ofMassosporaspp. are not well understood. The acquisition ofM. diceroproctaefrom Arizona,M. tettigatisfrom Chile, andM. platypediaefrom California and Colorado provided an opportunity to conduct molecular phylogenetic analyses and morphological studies to investigate if these fungi represent a monophyletic group and delimit species boundaries. In a three-locus phylogenetic analysis including the D1–D2 domains of the nuclear 28S rRNA gene (28S),elongation factor 1 alpha-like(EFL), andbeta-tubulin(BTUB),Massosporawas resolved in a strongly supported monophyletic group containing four well-supported genealogically exclusive lineages, based on two of three methods of phylogenetic inference. There was incongruence among the single-gene trees: two methods of phylogenetic inference recovered trees with either the same topology as the 3-gene concatenated tree (EFL), or a basal polytomy (28S, BTUB).Massospora levisporaandM. platypediaeisolates formed a single lineage in all analyses and are synonymized here asM. levispora.Massospora diceroproctaewas sister toM. cicadinain all three single-gene trees and on an extremely long branch relative to the otherMassospora, and even the outgroup taxa, which may reflect an accelerated rate of molecular evolution and/or incomplete taxa sampling. The results of the morphological study presented here indicate that spore measurements may not be phylogenetically or diagnostically informative. Despite recent advances in understanding the ecology ofMassospora, much about its host range and diversity remains unexplored. The emerging phylogenetic framework can provide a foundation for exploring co-evolutionary relationships with cicada hosts and the evolution of behavior-altering compounds. |
| Databáze: | OpenAIRE |
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