The intranasal vaccination of pregnant dams with Intimin and EspB confers protection in neonatal mice from Escherichia coli (EHEC) O157:H7 infection

Autor: E.C. Mercado, B.C. Rabinovitz, Ángel Adrián Cataldi, Mariano Larzabal, Daniel A. Vilte
Rok vydání: 2016
Předmět:
0301 basic medicine
VACCINE
Escherichia coli O157
medicine.disease_cause
Microbiology
Ciencias Biológicas
Mice
03 medical and health sciences
Biología Celular
Microbiología
Pregnancy
medicine
Animals
Weaning
ESPB
Adhesins
Bacterial
Escherichia coli
Administration
Intranasal
Escherichia coli Infections
Intimin
Mice
Inbred BALB C
General Veterinary
General Immunology and Microbiology
biology
Escherichia coli Vaccines
Escherichia coli Proteins
Public Health
Environmental and Occupational Health
biochemical phenomena
metabolism
and nutrition
Antibodies
Bacterial
Recombinant Proteins
Vaccination
Titer
030104 developmental biology
Infectious Diseases
Immunization
Immunoglobulin G
Immunology
biology.protein
EHEC
bacteria
Molecular Medicine
Female
Nasal administration
INTIMIN
Antibody
Immunity
Maternally-Acquired
CIENCIAS NATURALES Y EXACTAS
Bacterial Outer Membrane Proteins
Zdroj: Vaccine. 34:2793-2797
ISSN: 0264-410X
Popis: Enterohemorrhagic Escherichia coli (EHEC) O157:H7 is responsible for intestinal disease and hemolytic uremic syndrome (HUS), a serious systemic complication which particularly affects children. In this study, we evaluated whether passive immunization protects from EHEC O157:H7 colonization and renal damage, by using a weaned BALB/c mouse model of infection. Recombinant proteins EspB and the carboxyl-terminal fragment of 280 amino acids of -intimin (-Int C280) were used in combination with a macrophage-activating lipopeptide-2 (MALP) adjuvant to immunize pregnant mice by the intranasal route. Neonatal mice were allowed to suckle vaccinated or sham-vaccinated dams until weaning when they were challenged by the oral route with a suspension of an E. coli O157:H7 Stx2+ strain. The excretion ofthe inoculated strain was followed for 72 h. All vaccinated dams exhibited elevated serum IgG response against both -Int C280 and EspB. Passive immunization of newborn mice resulted in a significantincrease in serum IgG titers against -Int C280 and a slight increase in EspB-specific antibodies. The neonates from vaccinated dams showed a significant reduction in EHEC O157:H7 colonization 48 h post challenge. In addition, the level of plasma urea concentration, a marker of renal failure, was significantly higher in offsprings of sham-vaccinated mice. In conclusion, vaccination of pregnant dams with -Int C280 and EspB could reduce colonization and systemic toxicity of EHEC O157:H7 in their suckling offsprings. Fil: Rabinovitz, Bettina Carol. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Larzabal, Mariano. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Vilte, Daniel Alejandro. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina Fil: Cataldi, Ángel Adrián. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Mercado, Elsa Cristina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Databáze: OpenAIRE
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