Selenium status as determinant of connexin-43 dephosphorylation in ex vivo ischemic/reperfused rat myocardium
| Autor: | Corinne Berthonneche, Véronique Ducros, Andry Rakotovao, Arpad Tosaki, Joël de Leiris, François Boucher, Stéphane Tanguy, Marie Claire Toufektsian |
|---|---|
| Přispěvatelé: | Physiologie cardio-Respiratoire Expérimentale Théorique et Appliquée (TIMC-IMAG-PRETA), Techniques de l'Ingénierie Médicale et de la Complexité - Informatique, Mathématiques et Applications, Grenoble - UMR 5525 (TIMC-IMAG), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF), Physiopathologie des adaptations cardiovasculaires à l'Exercice, Avignon Université (AU), Département de biologie intégrée, CHU Grenoble-Hôpital Michallon, Pharmacology, University of Debrecen, Andry Rakotovao was supported by a grant from 'Fondation Robert Schumann'. Stéphane Tanguy was supported by the 'Centre Evian pour l'Eau'. |
| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: |
Male
MESH: Selenium Antioxidant medicine.medical_treatment MESH: Random Allocation MESH: Gap Junctions Myocardial Ischemia Connexin 030204 cardiovascular system & hematology medicine.disease_cause Biochemistry Random Allocation 0302 clinical medicine MESH: Myocardial Reperfusion MESH: Animals Phosphorylation chemistry.chemical_classification 0303 health sciences MESH: Oxidative Stress Glutathione peroxidase Gap Junctions Heart Orvostudományok MESH: Myocardial Reperfusion Injury Reperfusion injury MESH: Arrhythmias Cardiac medicine.anatomical_structure Cardiology Molecular Medicine Connexin-43 MESH: Myocardial Ischemia medicine.medical_specialty MESH: Myocardium MESH: Rats Ischemia Myocardial Reperfusion Myocardial Reperfusion Injury In Vitro Techniques Inorganic Chemistry Dephosphorylation 03 medical and health sciences Selenium MESH: Diet [SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system Internal medicine medicine Animals Rats Wistar 030304 developmental biology Glutathione Peroxidase MESH: Phosphorylation business.industry Myocardium Gyógyszerészeti tudományok Arrhythmias Cardiac MESH: Rats Wistar medicine.disease MESH: Connexin 43 MESH: Male Diet Rats Oxidative Stress Endocrinology chemistry Ventricle Connexin 43 MESH: Glutathione Peroxidase business Oxidative stress |
| Zdroj: | Journal of Trace Elements in Medicine and Biology Journal of Trace Elements in Medicine and Biology, Elsevier, 2005, 19 (1), pp.43-7. ⟨10.1016/j.jtemb.2005.07.001⟩ |
| ISSN: | 0946-672X 1878-3252 |
| DOI: | 10.1016/j.jtemb.2005.07.001⟩ |
| Popis: | International audience; Recent studies have demonstrated that electrical uncoupling at gap junctions during ischemia is associated with cardiac Connexin-43 (Cx43) dephosphorylation. Whether oxidative stress is involved in this phenomenon still remains unclear. In the present study, we examined the influence of selenium intake on reperfusion-induced Cx43 dephosphorylation. Male Wistar rats were fed a diet containing either 0.05 mg/kg (Low-Se, n = 13) or 1.5 mg/kg (High-Se, n = 11) selenium for 8 weeks. At the end of this diet, hearts were isolated and subjected to 10 min regional ischemia followed by 10 min reperfusion. The level of dephosphorylated Cx43 was determined in tissue samples from ischemic/reperfused and non-ischemic regions of the hearts. At the end of the experiemental diet, the activity of the antioxidant enzyme glutathione peroxidase (GSH-Px) was increased in high-Se hearts compared with low-Se hearts (+ 13%; p < 0.05). After ischemia/reperfusion, in low-Se hearts, Cx43 dephosphorylation appeared significantly increased in the left ventricle compared to the non-ischemic right ventricle (+ 149%; p < 0.05). The high-Se diet significantly reduced Cx43 dephosphorylation in the left ventricle (p < 0.05 vs. low-Se diet). In conclusion, our results suggest that oxidative stress may be involved in Cx43 dephosphorylation during myocardial ischemia/reperfusion, thereby contributing to arrhythmogenesis. |
| Databáze: | OpenAIRE |
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