d-Ribose contributes to the glycation of serum protein
| Autor: | Lexiang Yu, Yong Xu, Yan Wei, Yao Chen, Tao He, Rongqiao He, Yujing Wang |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Glycation End Products
Advanced Male 0301 basic medicine medicine.medical_specialty Glycosylation Ribose medicine.medical_treatment Intraperitoneal injection Lysine Serum Albumin Human Diabetes Mellitus Experimental Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine stomatognathic system Tandem Mass Spectrometry In vivo D-Glucose Glycation Internal medicine Diabetes mellitus medicine Animals Humans Molecular Biology Chromatography High Pressure Liquid Glycopeptides medicine.disease Human serum albumin Rats Kinetics Glucose 030104 developmental biology Endocrinology Diabetes Mellitus Type 2 chemistry 030220 oncology & carcinogenesis Molecular Medicine medicine.drug |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease. 1865:2285-2292 |
| ISSN: | 0925-4439 |
| Popis: | d -Ribose is active in glycation and rapidly produces advanced glycation end products, leading to cell death and to cognitive impairment in mice. Glycated serum protein (GSP) is a relatively short-term biomarker for glycemic control in diabetes mellitus. However, whether d -ribose is related to GSP is unclear. The aim of this work was to identify the contribution of d -ribose to GSP compared to d -glucose. Here, we showed that the yield of glycated human serum albumin with d -ribose was at least two-fold higher than that with d -glucose in a 2-week incubation. The glycation of human serum albumin (HSA) with d -ribose was much faster than that with d -glucose, as determined by monitoring changes in the fluorescent intensity of glycation products with time. Liquid chromatography-mass spectrometry/mass spectrometry revealed that 17 and 7 lysine residues on HSA were glycated in the presence of d -ribose and d -glucose, respectively, even when the concentration ratio [ d -ribose]/[ d -glucose] was 1/50. The intraperitoneal injection of d -ribose significantly increased the GSP levels in Sprague Dawley rats, but the injection of d -glucose did not. The level of d -ribose was more positively associated with GSP than the level of d -glucose in streptozotocin-treated rats. In diabetic patients, the levels of both d -ribose and d -glucose were closely related to the level of GSP. Together, these in vitro and in vivo findings indicated that d -ribose is an important contributor to the glycation of serum protein, compared to d -glucose. To assess GSP levels in diabetes mellitus, we should consider the contribution from d -ribose, which plays a nonnegligible role. |
| Databáze: | OpenAIRE |
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