An O antigen can interfere with the function of the Yersinia pseudotuberculosis invasin protein
| Autor: | Ralph R. Isberg, D. L. Voorhis, S. Dillon, S. B. Formal |
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| Rok vydání: | 1991 |
| Předmět: |
Virulence
medicine.disease_cause Microbiology Shigella flexneri Plasmid Antigen Bacterial Proteins medicine Tumor Cells Cultured Yersinia pseudotuberculosis Humans Adhesins Bacterial Molecular Biology Escherichia coli Gene Antigens Bacterial biology O Antigens biochemical phenomena metabolism and nutrition biology.organism_classification Enterobacteriaceae Phenotype Antigens Surface |
| Zdroj: | Molecular microbiology. 5(2) |
| ISSN: | 0950-382X |
| Popis: | Escherichia coli strains harbouring the Yersinia pseudotuberculosis inv gene are able to enter cultured mammalial cells. We show here that this property is not shared by all enteric bacteria, since Shigella flexneri 2a cured of its virulence-associated plasmid and harbouring the inv gene is unable to enter mammalian cells efficiently. Mapping studies showed that the region of the chromosome responsible for this phenotype includes rfaB, a locus involved in the production of O antigen. S. flexneri 2a strains that express O antigen were unable to enter mammalian cells, even though invasin was efficiently expressed and localized, showing that this structure interferes with invasin activity. The O antigen either masks invasin or sterically hinders the ability of the mammalian cell receptor to bind this protein. |
| Databáze: | OpenAIRE |
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