A barrier against reactive oxygen species: chitosan/acellular dermal matrix scaffold enhances stem cell retention and improves cutaneous wound healing

Autor:	Wenjing Guo, Wei Lin, Baoping Lin, Heting Chen, Xiaoyang Qi, Xiaoyuan Deng, Danyang Liang
Jazyk:	angličtina
Rok vydání:	2020
Předmět:	0301 basic medicine Cell type Chitosan/Acellular dermal matrix scaffold Medicine (miscellaneous) Wound healing 02 engineering and technology medicine.disease_cause Biochemistry Genetics and Molecular Biology (miscellaneous) lcsh:Biochemistry 03 medical and health sciences Paracrine signalling Stem cell retention medicine Acellular Dermis lcsh:QD415-436 chemistry.chemical_classification Chitosan Reactive oxygen species lcsh:R5-920 Stem Cells Research Mesenchymal stem cell Cell Biology 021001 nanoscience & nanotechnology Cell biology ROS barrier in inflammatory response 030104 developmental biology chemistry Molecular Medicine Stem cell Reactive Oxygen Species 0210 nano-technology lcsh:Medicine (General) Intracellular Oxidative stress
Zdroj:	Stem Cell Research & Therapy, Vol 11, Iss 1, Pp 1-17 (2020) Stem Cell Research & Therapy
ISSN:	1757-6512
DOI:	10.1186/s13287-020-01901-6
Popis:	Background Stem cell therapies have gained great attention for providing novel solutions for treatment of various injuries and diseases due to stem cells’ self-renewal, ability to differentiate into various cell types, and favorite paracrine function. Nevertheless, the low retention of transplanted stem cell still limits their clinical applications such as in wound healing in view of an induced harsh microenvironment rich in reactive oxygen species (ROS) during inflammatory reactions. Methods Herein, a novel chitosan/acellular dermal matrix (CHS/ADM) stem cell delivery system is developed, which is of great ROS scavenging activity and significantly attenuates inflammatory response. Result Under ROS microenvironment, this stem cell delivery system acts as a barrier, effectively scavenging an amount of ROS and protecting mesenchymal stem cells (MSCs) from the oxidative stress. It notably regulates intracellular ROS level in MSCs and reduces ROS-induced cellular death. Most importantly, such MSCs delivery system significantly enhances in vivo transplanted stem cell retention, promotes the vessel growth, and accelerates wound healing. Conclusions This novel delivery system, which overcomes the limitations of conventional plain collagen-based delivery system in lacking of ROS-environmental responsive mechanisms, demonstrates a great potential use in stem cell therapies in wound healing.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d454812affef74260407ca3594bb8be1 http://link.springer.com/article/10.1186/s13287-020-01901-6 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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