Photo-Triggered Click Chemistry for Biological Applications
| Autor: | Qing Lin, András Herner |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Azides
Light Chemical biology Tetrazoles Nanotechnology Alkenes 010402 general chemistry 01 natural sciences Article Bacterial protein Light source Bacterial Proteins RNA Transfer Organic chemistry Animals Metal catalyst Cycloaddition Reaction 010405 organic chemistry Chemistry General Chemistry Limiting 0104 chemical sciences Light intensity Click chemistry Click Chemistry Bioorthogonal chemistry |
| Zdroj: | Cycloadditions in Bioorthogonal Chemistry ISBN: 9783319296845 |
| DOI: | 10.1007/978-3-319-29686-9_5 |
| Popis: | In the last decade and a half, numerous bioorthogonal reactions have been developed with a goal to study biological processes in their native environment, i.e., in living cells and animals. Among them, the photo-triggered reactions offer several unique advantages including operational simplicity with the use of light rather than toxic metal catalysts and ligands, and exceptional spatiotemporal control through the application of an appropriate light source with pre-selected wavelength, light intensity and exposure time. While the photoinduced reactions have been studied extensively in materials research, e.g., on macromolecular surface, the adaptation of these reactions for chemical biology applications is still in its infancy. In this chapter, we review the recent efforts in the discovery and optimization the photo-triggered bioorthogonal reactions, with a focus on those that have shown broad utility in biological systems. We discuss in each cases the chemical and mechanistic background, the kinetics of the reactions and the biological applicability together with the limiting factors. |
| Databáze: | OpenAIRE |
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