Silencing circular RNA VANGL1 inhibits progression of bladder cancer by regulating miR‐1184/IGFBP2 axis

Autor: Dong Li, Shutian Zhao, Haining Qian, Bingyan Liu, An Xu, Dengke Yang, Zhen Fang
Rok vydání: 2019
Předmět:
0301 basic medicine
Cancer Research
Small interfering RNA
Mice
Nude
Apoptosis
medicine.disease_cause
lcsh:RC254-282
Mice
03 medical and health sciences
0302 clinical medicine
Circular RNA
microRNA
Biomarkers
Tumor
Tumor Cells
Cultured
medicine
metastasis
Animals
Humans
Gene silencing
Radiology
Nuclear Medicine and imaging
Original Research
Cancer Biology
Cell Proliferation
Mice
Inbred BALB C
Chemistry
Cell growth
Membrane Proteins
RNA
Circular
Transfection
lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens
Prognosis
microenvironment
Xenograft Model Antitumor Assays
Gene Expression Regulation
Neoplastic
MicroRNAs
Insulin-Like Growth Factor Binding Protein 3
030104 developmental biology
Urinary Bladder Neoplasms
Oncology
030220 oncology & carcinogenesis
Cancer research
Carrier Proteins
Carcinogenesis
Immortalised cell line
signal transduction
Zdroj: Cancer Medicine
Cancer Medicine, Vol 9, Iss 2, Pp 700-710 (2020)
ISSN: 2045-7634
DOI: 10.1002/cam4.2650
Popis: Circular RNA VANGL1 (circVANGL1) is generated from two exons of the Van Gogh‐like 1 (VANGL1) gene and serves as a tumor promoter by sponging certain microRNAs (miRNAs). However, the role of circVANGL1 in bladder cancer (BC) is still unclear. So, in order to investigate the role of circVANGL1 in BC, quantitative reverse transcription‐polymerase chain reaction (qRT‐PCR) was employed to evaluate the circVANGL1 expression in tumor tissues from BC patients and in BC cell lines. Small interfering RNA against circVANGL1 was constructed and stably transfected into human bladder epithelium immortalized cells (SV‐HUC). Cell invasion and migration were detected in Transwell chambers, cell proliferation was determined by CCK8 assays, and tumorigenesis in nude mice was examined to assess the effect of circVANGL1 in BC. Subcellular localization of circVANGL1 was confirmed by fluorescence in situ hybridization. The interactive relationships among circVANGL1, miRNA, and relative proteins were confirmed by luciferase reporter assays. The results showed that circVANGL1 was upregulated in both BC tissues and cell lines. Silencing the expression of circVANGL1 suppressed cell invasion, migration, and proliferation during in vitro experiments. Mechanistically, we demonstrated that circVANGL1 upregulated the expression of miR‐1184 target gene insulin‐like growth factor‐binding protein 2 (IGFBP2) by sponging miR‐1184, which promoted the aggressive biological behaviors of BC. Taken together, our results indicate that circVANGL1 acts as a tumor promoter through the novel circVANGL1/miR‐1184/IGFBP2 axis. Hopefully, our study will provide new ideas for the clinical treatment of BC.
Circular RNA VANGL1 (circVANGL1) upregulated the expression of miR‐1184 target gene insulin‐like growth factor‐binding protein 2 (IGFBP2) by sponging miR‐1184, which promoted the aggressive biological behaviors of BC. Taken together, our results indicate that circVANGL1 acts as a tumor promoter through the novel circVANGL1/miR‐1184/IGFBP2 axis.
Databáze: OpenAIRE
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