A graphene-based glycan biosensor for electrochemical label-free detection of a tumor-associated antibody
| Autor: | Anna Blsakova, Peter Kasak, Jan Tkac, Monika Jerigova, Dusan Velic, Ola Blixt, Filip Kveton, Bo Jansson, Lenka Lorencova |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Analyte
Glycan Antibodies Neoplasm Biosensing Techniques 02 engineering and technology biosensor Electrochemistry 01 natural sciences Biochemistry Article Analytical Chemistry law.invention Limit of Detection law Tn antigen medicine Humans Antigens Tumor-Associated Carbohydrate Electrical and Electronic Engineering Surface plasmon resonance Electrodes graphene screen-printed electrodes Instrumentation Serum Albumin glycan biology 010405 organic chemistry Chemistry Graphene Graphene screen-printed electrodes technology industry and agriculture Electrochemical Techniques 021001 nanoscience & nanotechnology Human serum albumin Combinatorial chemistry Atomic and Molecular Physics and Optics 0104 chemical sciences carbohydrates (lipids) electrochemistry Covalent bond biology.protein Graphite 0210 nano-technology Biosensor medicine.drug |
| Zdroj: | Kveton, F, Blsakova, A, Lorencova, L, Jerigova, M, Velic, D, Blixt, O, Jansson, B, Kasak, P & Tkac, J 2019, ' A Graphene-Based Glycan Biosensor for Electrochemical Label-Free Detection of a Tumor-Associated Antibody ', Sensors, vol. 19, no. 24, 5409, pp. 1-13 . https://doi.org/10.3390/s19245409 Sensors Volume 19 Issue 24 Sensors (Basel, Switzerland) |
| DOI: | 10.3390/s19245409 |
| Popis: | The study describes development of a glycan biosensor for detection of a tumor-associated antibody. The glycan biosensor is built on an electrochemically activated/oxidized graphene screen-printed electrode (GSPE). Oxygen functionalities were subsequently applied for covalent immobilization of human serum albumin (HSA) as a natural nanoscaffold for covalent immobilization of Thomsen-nouvelle (Tn) antigen (GalNAc-O-Ser/Thr) to be fully available for affinity interaction with its analyte&mdash a tumor-associated antibody. The step by step building process of glycan biosensor development was comprehensively characterized using a battery of techniques (scanning electron microscopy, atomic force microscopy, contact angle measurements, secondary ion mass spectrometry, surface plasmon resonance, Raman and energy-dispersive X-ray spectroscopy). Results suggest that electrochemical oxidation of graphene SPE preferentially oxidizes only the surface of graphene flakes within the graphene SPE. Optimization studies revealed the following optimal parameters: activation potential of +1.5 V vs. Ag/AgCl/3 M KCl, activation time of 60 s and concentration of HSA of 0.1 g L&minus 1. Finally, the glycan biosensor was built up able to selectively and sensitively detect its analyte down to low aM concentration. The binding preference of the glycan biosensor was in an agreement with independent surface plasmon resonance analysis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|