Effects of Aspirin on Arylamine N-Acetyltransferase Activity in Klebsiella Pneumoniae
| Autor: | Jing Gung Chung, Jem Mi Lai, Wen Tsong Hsieh, Guang Wei Chen, Huei Yann Tsai, Tzu Wei Tan |
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| Rok vydání: | 1998 |
| Předmět: |
Time Factors
Arylamine N-Acetyltransferase Klebsiella pneumoniae Health Toxicology and Mutagenesis Pharmacology Toxicology Microbiology Cytosol medicine Chromatography High Pressure Liquid Arylamine N-acetyltransferase activity chemistry.chemical_classification Fluorenes Aspirin Chemical Health and Safety biology Arylamine N-acetyltransferase Public Health Environmental and Occupational Health Acetylation General Medicine biology.organism_classification Enterobacteriaceae Enzyme chemistry Toxicity medicine.drug |
| Zdroj: | Drug and Chemical Toxicology. 21:507-520 |
| ISSN: | 1525-6014 0148-0545 |
| DOI: | 10.3109/01480549809002219 |
| Popis: | This study was designed to assess the effects of aspirin on arylamine N-acetyltransferase (NAT) activities in the bacterium Klebsiella pneumoniae using high performance liquid chromatography to measure the acetylation of 2-aminofluorene (2-AF) with or without aspirin. Cytosols or suspensions of K. pneumoniae with or without specific concentrations of aspirin co-treatment showed different percentages of 2-AF acetylation. The data indicated that there was decreased NAT activity associated with increased levels of aspirin in K. pneumoniae cytosols and in intact bacteria. For the cytosol examination, the apparent values of Km and Vmax decreased 0.59- and 0.58-fold after co-treated with 40 microM aspirin, respectively, for 2-AF. For the intact bacteria examination, the apparent values of Km and Vmax decreased 0.60- and 0.67-fold after co-treated with 40 microM aspirin, respectively, for 2-AF. This report is the first demonstration to show that aspirin can decrease N-acetyltransferase activity in the bacterium K. pneumoniae. |
| Databáze: | OpenAIRE |
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