Rice Breeding in Russia Using Genetic Markers
| Autor: | Sergey Lesnyak, Pavel Kostylev, Kirill Azarin, Elena Krasnova, Margarita G. Ruban, Elena Dubina |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Soil salinity Introgression Locus (genetics) Plant Science blast Biology 01 natural sciences Article salinity 03 medical and health sciences PCR analysis Genotype Gene Ecology Evolution Behavior and Systematics Hybrid SSR markers Ecology business.industry rice Botany food and beverages Biotechnology 030104 developmental biology Genetic marker Agriculture QK1-989 submergence tolerance business 010606 plant biology & botany |
| Zdroj: | Plants, Vol 9, Iss 1580, p 1580 (2020) Plants Volume 9 Issue 11 |
| ISSN: | 2223-7747 |
| Popis: | The article concentrates on studying tolerance to soil salinization, water flooding, and blast in Russian and Asian rice varieties, as well as hybrids of the second and third generations from their crossing in order to obtain sustainable paddy crops based on domestic varieties using DNA markers. Samples IR 52713-2B-8-2B-1-2, IR 74099-3R-3-3, and NSIC Rc 106 were used as donors of the SalTol tolerance gene. Varieties with the Sub1A locus were used as donors of the flood resistance gene: Br-11, CR-1009, Inbara-3, TDK-1, and Khan Dan. The lines C101-A-51 (Pi-2), C101-Lac (Pi-1, Pi-33), IR-58 (Pi-tа), and Moroberekan (Pi-b) were used to transfer blast resistance genes. Hybridization of the stress-sensitive domestic varieties Novator, Flagman, Virazh, and Boyarin with donor lines of the genes of interest was carried out. As a result of the studies carried out using molecular marking based on PCR in combination with traditional breeding, early-maturing rice lines with genes for resistance to salinity (SalTol) and flooding (Sub1A), suitable for cultivation in southern Russia, were obtained. Introgression and pyramiding of the blast resistance genes Pi-1, Pi-2, Pi-33, Pi-ta, and Pi-b into the genotypes of domestic rice varieties were carried out. DNA marker analysis revealed disease-resistant rice samples carrying 5 target genes in a homozygous state. The created rice varieties that carry the genes for blast resistance (Pentagen, Magnate, Pirouette, Argamac, Kapitan, and Lenaris) were submitted for state variety testing. The introduction of such varieties into production will allow us to avoid epiphytotic development of the disease, preserving the biological productivity of rice and obtaining environmentally friendly agricultural products. |
| Databáze: | OpenAIRE |
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