SCID/NOD mice model for 5-FU induced intestinal mucositis: Safety and effects of probiotics as therapy
| Autor: | Chun-Yan Yeung, Jen-Shiu Chiang Chiau, Hung-Chang Lee, Wai-Tao Chan, Mei-Lien Cheng, Chuen-Bin Jiang, Szu-Wen Chang, Lawrence Huang |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Male
Mucositis 0301 basic medicine Lactobacillus casei medicine.medical_specialty ved/biology.organism_classification_rank.species Mice SCID Gastroenterology law.invention Mice 03 medical and health sciences Probiotic 0302 clinical medicine Lactobacillus acidophilus Mice Inbred NOD law Internal medicine medicine Animals Intestinal Mucosa NOD mice Bifidobacterium bifidum biology ved/biology business.industry Probiotics Weight change lcsh:RJ1-570 lcsh:Pediatrics biology.organism_classification medicine.disease Disease Models Animal Diarrhea 030104 developmental biology 030220 oncology & carcinogenesis Pediatrics Perinatology and Child Health Cytokines Fluorouracil medicine.symptom business |
| Zdroj: | Pediatrics and Neonatology, Vol 60, Iss 3, Pp 252-260 (2019) |
| ISSN: | 1875-9572 |
| DOI: | 10.1016/j.pedneo.2018.07.007 |
| Popis: | Background: For chemotherapy patients, intestinal mucositis is a frequent complication. Previously, we evaluated the beneficial effect of oral probiotics in 5-Fluorouracil (5-FU) induced mucositis in BALB/c mice. Here, we used SCID/NOD mice instead to simulate the immunodeficiency of chemotherapy patients: first, to evaluate the safety of probiotic supplementation and second, to determine the probiotic effect in response to 5-FU intestinal mucositis. Methods: Thirty-six SCID/NOD mice were injected with saline (three control groups) or 5-FU (three experimental groups) intraperitoneally daily for five days. Mice were given either oral saline daily, probiotic suspension of Lactobacillus casei variety rhamnosus (Lcr35, Antibiophilus™, France) or Lactobacillus acidophilus and Bifidobacterium bifidum (LaBi, Infloran™, Italy). Blood, liver, spleen, and lymph node tissue samples were evaluated for probiotic translocation via culture and Q-PCR. Weight change, diarrhea score, jejunal villus height (VH) and crypt depth (CD), and serum cytokine levels of TNF-α, IFNγ, IL-1β, IL-6, IL-4, IL-10, IL-13, and IL-17 were also assessed. Results: No weight loss was found in the SCID control group. Mean weight loss of 10.63 ± 0.87% was noted by day five in 5-FU group without probiotics but it was only 6.2 ± 0.43% if mice were given Lcr35 (p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|