Measuring NAD+levels in mouse blood and tissue samples via a surrogate matrix approach using LC–MS/MS
| Autor: | Yuzhong Deng, Cornelis E. C. A. Hop, Lulu Yang, Xiaorong Liang, Mark Zak, Kirsten Messick, Shuguang Ma, Ann R Qin, Peter S. Dragovich, Justin Ly, Bianca M. Liederer, Brian Dean |
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| Rok vydání: | 2014 |
| Předmět: |
Blood Specimen Collection
Analyte High-throughput screening Clinical Biochemistry Nicotinamide phosphoribosyltransferase Endogeny General Medicine Biology NAD Mass Spectrometry Analytical Chemistry Matrix (chemical analysis) Mice Medical Laboratory Technology chemistry.chemical_compound chemistry Biochemistry Oral administration Animals NAD+ kinase Sample collection General Pharmacology Toxicology and Pharmaceutics Chromatography Liquid |
| Zdroj: | Bioanalysis. 6:1445-1457 |
| ISSN: | 1757-6199 1757-6180 |
| Popis: | Background: NAD+is an endogenous analyte and is unstable during blood sample collection, both of which present obstacles for quantitation. Moreover, current procedures for NAD+sample collection require onsite treatment with strong acid to stabilize the NAD+in mouse blood cells. Results: NAD+can be stabilized by addition of acid before the frozen mouse blood sample was thawed. A simple sample collection procedure was proposed to facilitate the analysis of NAD+in mouse blood and tissue samples. A LC–MS/MS method was developed for quantifying NAD+in mouse blood and various tissue samples. The described method was used to measure endogenous NAD+levels in mouse blood following oral administration of the nicotinamide phosphoribosyltransferase inhibitor GNE-617. Conclusion: This study presents a suitable assay and sample collection procedure for high throughput screening of NAD+samples in preclinical discovery studies. |
| Databáze: | OpenAIRE |
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