Assessing the clinical value of microRNAs in formalin-fixed paraffin-embedded liposarcoma tissues: Overexpressed miR-155 is an indicator of poor prognosis
| Autor: | Evangelos Briasoulis, Penelope Gogou, Anna Batistatou, Konstantinos Mavridis, Andreas Scorilas, Ioannis Sainis, Nikolaos Kapodistrias, Vasilios Karavasilis |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Oncology Male Pathology Time Factors Tissue Fixation Kaplan-Meier Estimate 0302 clinical medicine prognostic biomarker Aged 80 and over Paraffin Embedding Reverse Transcriptase Polymerase Chain Reaction Liposarcoma Lipoma Middle Aged 3. Good health Up-Regulation Gene Expression Regulation Neoplastic Treatment Outcome 030220 oncology & carcinogenesis Area Under Curve miRNAs Disease Progression Female RNA extraction Sarcoma Research Paper Adult medicine.medical_specialty Malignancy Disease-Free Survival miR-155 03 medical and health sciences Fixatives Young Adult Predictive Value of Tests Internal medicine Formaldehyde microRNA medicine Biomarkers Tumor Humans miRNA expression normalization Aged Proportional Hazards Models business.industry medicine.disease Reverse transcriptase MicroRNAs 030104 developmental biology ROC Curve Neoplasm Grading business |
| Zdroj: | Oncotarget |
| ISSN: | 1949-2553 |
| Popis: | // Nikolaos Kapodistrias 1 , Konstantinos Mavridis 2 , Anna Batistatou 3 , Penelope Gogou 4 , Vasilios Karavasilis 5 , Ioannis Sainis 1 , Evangelos Briasoulis 1 , Andreas Scorilas 2 1 Cancer Biobank Center, University of Ioannina, University Campus, Ioannina, Greece 2 Department of Biochemistry and Molecular Biology, National and Kapodistrian University of Athens, Panepistimiopolis, Athens, Greece 3 Department of Pathology, School of Medicine, University of Ioannina, Greece 4 Clinical Oncology Department, Norwich University Hospital, UK 5 London Sarcoma Service, Department of Oncology, UCLH, London, UK Correspondence to: Andreas Scorilas, email: ascorilas@biol.uoa.gr Keywords: liposarcoma, miRNAs, miR-155, miRNA expression normalization, prognostic biomarker Received: July 05, 2016 Accepted: December 01, 2016 Published: December 28, 2016 ABSTRACT Liposarcoma (LPS) is a malignancy with extreme heterogeneity and thus optimization towards personalizing patient prognosis and treatment is essential. Here, we evaluated miR-155, miR-21, miR-143, miR-145 and miR-451 that are implicated in LPS, as novel FFPE tissue biomarkers. A total of 83 FFPE tissue specimens from primary LPS and lipomas (LPM) were analyzed. A proteinase K incubation-Trizol treatment coupled protocol was used for RNA isolation. After polyadenylation of total RNA and reverse transcription, expression analysis of 9 candidate reference and 5 target miRNAs was performed by qPCR. Genorm and NormFinder were used for finding the most suitable molecules for normalization. Survival analyses were performed in order to evaluate the prognostic potential of miRNAs. MiR-103 and miR-191 are most suitable for normalization of miRNA expression in LPS. MiR-155 and miR-21 are clearly overexpressed (P |
| Databáze: | OpenAIRE |
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