Pitfalls in the characterization of circulating and tissue-resident human γδ T cells
| Autor: | Susanne Sebens, Markus W. Löffler, Jonas Bochem, Kilian Wistuba-Hamprecht, Christian Peters, Daniela Wesch, Dieter Kabelitz, Paola Nocerino, Hisayoshi Hashimoto, Hans-Heinrich Oberg, Karin Schilbach, Claus Garbe, Christoph Röcken, Shahram Kordasti, Nicola Beucke, Graham Pawelec, Benjamin Weide |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
mass cytometry T cell Immunology Gene Expression Biology γδ T cells Antibodies Flow cytometry 03 medical and health sciences 0302 clinical medicine magnetic cell isolation T-Lymphocyte Subsets immunomonitoring medicine Immunology and Allergy Humans Mass cytometry Cell isolation Fluorescent Dyes medicine.diagnostic_test Immunomagnetic Separation flow cytometry Carcinoma Receptors Antigen T-Cell gamma-delta Cell Biology Flow Cytometry Hodgkin Disease Immunohistochemistry 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis Case-Control Studies Colonic Neoplasms immunohistochemistry Leukocytes Mononuclear |
| Zdroj: | Beucke, N, Wesch, D, Oberg, H-H, Peters, C, Bochem, J, Weide, B, Garbe, C, Pawelec, G, Sebens, S, Röcken, C, Hashimoto, H, Löffler, M W, Nocerino, P, Kordasti, S, Kabelitz, D, Schilbach, K & Wistuba-Hamprecht, K 2020, ' Pitfalls in the characterization of circulating and tissue-resident human γδ T cells ', Journal of Leukocyte Biology, vol. 107, no. 6, pp. 1097-1105 . https://doi.org/10.1002/JLB.5MA1219-296R |
| DOI: | 10.1002/JLB.5MA1219-296R |
| Popis: | Dissection of the role and function of human γδ T cells and their heterogeneous subsets in cancer, inflammation, and auto-immune diseases is a growing and dynamic research field of increasing interest to the scientific community. Therefore, harmonization and standardization of techniques for the characterization of peripheral and tissue-resident γδ T cells is crucial to facilitate comparability between published and emerging research. The application of commercially available reagents to classify γδ T cells, in particular the combination of multiple Abs, is not always trouble-free, posing major demands on researchers entering this field. Occasionally, even entire γδ T cell subsets may remain undetected when certain Abs are combined in flow cytometric analysis with multicolor Ab panels, or might be lost during cell isolation procedures. Here, based on the recent literature and our own experience, we provide an overview of methods commonly employed for the phenotypic and functional characterization of human γδ T cells including advanced polychromatic flow cytometry, mass cytometry, immunohistochemistry, and magnetic cell isolation. We highlight potential pitfalls and discuss how to circumvent these obstacles. |
| Databáze: | OpenAIRE |
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