Cytogenetic status in newborns and their parents in Madrid: The BioMadrid study
Autor: | Amparo de Santos, Elisa Gil, Nuria Aragonés, J. Sanz, Gonzalo López-Abente, Virginia Lope, Jenaro Astray, Ángel Asensio, José Miguel García-Sagredo, María José González, Concha de Paz, Andrés Iriso, Marina Pollán, Mercedes Martínez, Ana María Pérez-Meixeira, José Frutos García, Margot Cisneros, América de León, Beatriz Pérez-Gómez, Mario Antonio Fernández |
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Rok vydání: | 2009 |
Předmět: |
Adult
Male Epidemiology Health Toxicology and Mutagenesis Binucleated cells Physiology Biology Elevated blood Arsenic Toxicology Pregnancy Biomonitoring medicine Humans Micronuclei Chromosome-Defective Genetics (clinical) Demography Micronucleus Tests Healthy population Infant Newborn Environmental Exposure Mercury medicine.disease Lead Socioeconomic Factors Spain Cytogenetic Analysis Micronucleus test Environmental Pollutants Female Population exposure Micronucleus Biomarkers Cadmium |
Zdroj: | Environmental and Molecular Mutagenesis. |
ISSN: | 1098-2280 0893-6692 |
Popis: | Monitoring cytogenetic damage is frequently used to assess population exposure to environmental mutagens. The cytokinesis-block micronucleus assay is one of the most widely used methods employed in these studies. In the present study we used this assay to assess the baseline frequency of micronuclei in a healthy population of father-pregnant woman-newborn trios drawn from two Madrid areas. We also investigated the association between micronucleus frequency and specific socioeconomic, environmental, and demographic factors collected by questionnaire. Mercury, arsenic, lead, and cadmium blood levels were measured by atomic absorption spectrometry. The association between micronucleated cell frequency and the variables collected by questionnaire, as well as, the risk associated with the presence of elevated levels of metals in blood, was estimated using Poisson models, taking the number of micronucleated cells in 1,000 binucleated cells (MNBCs) as the dependent variable. Separate analyses were conducted for the 110 newborns, 136 pregnant women, and 134 fathers in whom micronuclei could be assessed. The mean number of micronucleated cells per 1,000 binucleated cells was 3.9, 6.5, and 6.1 respectively. Our results show a statistically significant correlation in MNBC frequency between fathers and mothers, and between parents and newborns. Elevated blood mercury levels in fathers were associated with significantly higher MNBC frequency, compared with fathers who had normal mercury levels (RR:1.21; 95%CI:1.02-1.43). This last result suggests the need to implement greater control over populations which, by reason of their occupation or life style, are among those most exposed to this metal. |
Databáze: | OpenAIRE |
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