Intratunical injection of stromal vascular fraction prevents fibrosis in a rat model of Peyronie's disease

Autor:	Uros Milenkovic, Giulio Garaffa, Andrea Salonia, Lukman Hakim, Fabio Castiglione, Francesco Montorsi, Petter Hedlund, Steven Joniau, Maarten Albersen, Emmanuel Weyne, Dirk De Ridder, David Ralph, Trinity J. Bivalacqua, Asif Muneer
Přispěvatelé:	Castiglione, Fabio, Hedlund, Petter, Weyne, Emmanuel, Hakim, Lukman, Montorsi, Francesco, Salonia, Andrea, Bivalacqua, Trinity J, De Ridder, Dirk, Milenkovic, Uro, Ralph, David, Garaffa, Giulio, Muneer, Asif, Joniau, Steven, Albersen, Maarten
Rok vydání:	2019
Předmět:	collagen Male medicine.medical_specialty Mean arterial pressure erectile dysfunction extracellular matrix Urology Penile Induration 030232 urology & nephrology regenerative medicine Adipose tissue Injections Rats Sprague-Dawley Transforming Growth Factor beta1 03 medical and health sciences 0302 clinical medicine Fibrosis medicine Induration peni Animals penile curvature 030219 obstetrics & reproductive medicine biology business.industry adipose stem cell Histology Stromal vascular fraction medicine.disease penile plaque Rats Disease Models Animal inflammation sexual dysfunction biology.protein Stromal Cells Peyronie's disease business Elastin Transforming growth factor
Zdroj:	BJU International. 124:342-348
ISSN:	1464-4096
Popis:	Objective To investigate whether local injection of autologous adipose stromal vascular fraction (SVF) can prevent the development of fibrosis and elastosis in the tunica albuginea (TA) using a rat model of the acute phase of Peyronie's disease (PD). Methods A total of 24 male 12‐week‐old Sprague–Dawley rats were divided into three equal groups: sham; PD without treatment (transforming growth factor‐β [TGF ‐β]); and PD treated with SVF 1 day after disease induction. Sham rats received two injections of vehicle into the TA 1 day apart. TGF ‐β rats received TGF‐ β1 injection and injection of vehicle 1 day later. SVF rats received TGF‐β1 injection, followed by SVF 1 day later. One month after treatment, all rats underwent measurement of intracavernosal pressure and mean arterial pressure during electrostimulation of the cavernous nerve. The rats were then killed and penises were harvested for histology and Western blot analysis. Results Erectile function was moderately reduced in the TGF‐β group and was significantly improved after SVF treatment (P < 0.05). PD rats developed areas of fibrosis with a significant upregulation of collagen III, collagen I and elastin protein expression. These fibrotic changes were prevented when treated with SVF. Conclusions Local injection of SVF may represent treatment for the acute phase of PD.
Databáze:	OpenAIRE
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