Rare, high-affinity anti-pathogen antibodies from human repertoires, discovered using microfluidics and molecular genomics
| Autor: | Rena A. Mizrahi, Adam S. Adler, Matthew Adams, Michael A. Asensio, Robert C. Edgar, Jackson Leong, Renee Leong, David Goldblatt, Matthew J. Spindler, David S. Johnson, Lucy Roalfe, Rebecca White |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.drug_class Immunology Microfluidics Antibody Affinity Influenza A Monoclonal antibody medicine.disease_cause Affinity maturation 03 medical and health sciences deep sequencing 0302 clinical medicine Antigen Antibody Repertoire Anti-Infective Agents Peptide Library Report antibody repertoire medicine Influenza A virus Immunology and Allergy Humans Amino Acid Sequence Peptide library biology Antibodies Monoclonal High-Throughput Nucleotide Sequencing Genomics respiratory system Virology 3. Good health 030104 developmental biology Streptococcus pneumoniae 030220 oncology & carcinogenesis biology.protein Antibody pneumococcus Single-Chain Antibodies |
| Zdroj: | mAbs |
| ISSN: | 1942-0870 1942-0862 |
| Popis: | Affinity-matured, functional anti-pathogen antibodies are present at low frequencies in natural human repertoires. These antibodies are often excellent candidates for therapeutic monoclonal antibodies. However, mining natural human antibody repertoires is a challenge. In this study, we demonstrate a new method that uses microfluidics, yeast display, and deep sequencing to identify 247 natively paired anti-pathogen single-chain variable fragments (scFvs), which were initially as rare as 1 in 100,000 in the human repertoires. Influenza A vaccination increased the frequency of influenza A antigen-binding scFv within the peripheral B cell repertoire from |
| Databáze: | OpenAIRE |
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