SEPARATION OF RAT PLASMA HDL SUBFRACTIONS BY DENSITY GRADIENT CENTRIFUGATION AND THE EFFECT OF INCUBATION ON THESE FRACTIONS
| Autor: | Geesje M. Dallinga-Thie, G.C. Schoonderwoerd, Hans Jansen |
|---|---|
| Přispěvatelé: | Other departments |
| Jazyk: | angličtina |
| Rok vydání: | 1983 |
| Předmět: |
Apolipoprotein E
Very low-density lipoprotein Apolipoprotein B DTNB Cholesterol VLDL Biophysics Phospholipid Fractionation Lipoproteins VLDL Biochemistry chemistry.chemical_compound Endocrinology Centrifugation Density Gradient Animals Centrifugation Chromatography biology Chemistry Cholesterol Cholesterol HDL nutritional and metabolic diseases Rats Inbred Strains Cholesterol LDL Rats Lipoproteins LDL Kinetics Apolipoproteins biology.protein lipids (amino acids peptides and proteins) Female Lipoproteins HDL |
| Zdroj: | BIOCHIMICA ET BIOPHYSICA ACTA, 754(3), 271-278 |
| ISSN: | 0006-3002 |
| Popis: | The conditions for the separation of rat high density lipoproteins (HDL) in a single ultracentrifuge run are described. By this method six serum samples can be processed simultaneously. HDL is separated into two main fractions, one with apolipoprotein E and the other with apolipoprotein A-I as the major protein component. The apolipoprotein E-rich HDL contains a relatively high amount of phospholipid and unesterified cholesterol and therefore resembles HDL-1 or apolipoprotein E HDL as isolated by other methods. The other HDL fraction resembles HDL-2. The two HDL fractions appeared to be heterogeneous with respect to apolipoprotein composition. The HDL-1 consisted of particles with and without a low percentage of apolipoprotein A-I. The HDL-2 consisted of particles with a variable amount of apolipoprotein E and A-IV. During incubation of rat serum for 5 h at 37 degrees C in the presence of dithiobis(2-nitrobenzoic acid) (DTNB) a small shift of the HDL-2 peak to lower densities occurred. Incubation of the serum without DTNB led to a loss of cholesterol from the 'light' HDL-1 fractions and an increase in cholesterol ester in fractions at densities intermediate between those of HDL-1 and HDL-2 and in fractions at the densest part of the gradient. |
| Databáze: | OpenAIRE |
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