Effect of cadmium on the concentration of essential metals in a human chondrocyte micromass culture
| Autor: | Esther Aurora Ruiz-Huerta, Carlos Landa-Solís, M C Camacho-Rea, L Mendoza-Soto, Gabriela Angélica Martínez-Nava, Karina Martínez-Flores, Olivier Barbier, Anell Olivos-Meza, María Aurora Armienta-Hernández, D. Reyes-Hinojosa, L. M. Del Razo, Javier Fernández-Torres, E Hernández-Álvarez, Juana Narváez-Morales, Yessica Zamudio-Cuevas, C Suárez-Ahedo, R Plata-Rodríguez, B Vargas-Sandoval |
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| Rok vydání: | 2020 |
| Předmět: |
Adult
Male Iron Blotting Western chemistry.chemical_element Zinc Osteoarthritis 010501 environmental sciences 01 natural sciences Biochemistry Mass Spectrometry Chondrocyte Immunophenotyping Inorganic Chemistry Young Adult 03 medical and health sciences Chromium Chondrocytes 0302 clinical medicine Western blot Nickel medicine Humans 0105 earth and related environmental sciences Cadmium medicine.diagnostic_test Cartilage homeostasis Cartilage medicine.disease Molecular biology medicine.anatomical_structure chemistry Molecular Medicine 030217 neurology & neurosurgery |
| Zdroj: | Journal of Trace Elements in Medicine and Biology. 62:126614 |
| ISSN: | 0946-672X |
| DOI: | 10.1016/j.jtemb.2020.126614 |
| Popis: | Background An essential element imbalance in the joint might favor gradual degeneration of the articular cartilage. It has been reported that cadmium (Cd) plays an antagonistic role with regards to the presence of essential elements, such as zinc (Zn), iron (Fe), and manganese (Mn), which may favor the development of disabling diseases, like osteoarthritis (OA) and osteoporosis. Methods 3D cultures of human chondrocytes were phenotyped with the Western blot technique and structurally evaluated with histological staining. The samples were exposed to 1, 5, and 10 μM of CdCl2 for 12 h, with a non-exposed culture as control. The concentration of Cd, Fe, Mn, Zn, chromium (Cr), and nickel (Ni) was quantified through plasma mass spectrometry (ICP-MS). The data were analyzed with a Kruskal Wallis test, a Kendall’s Tau test and Spearman’s correlation coefficient with the Stata program, version 14. Results Our results suggest that Cd exposure affects the structure of micromass cultures and plays an antagonistic role on the concentration of essential metals, such as Zn, Ni, Fe, Mn, and Cr. Conclusion Cd exposure may be a risk factor for developing joint diseases like OA, as it can interfere with cartilage absorption of other essential elements that maintain cartilage homeostasis. |
| Databáze: | OpenAIRE |
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