Cyclodextrin production from amaranth starch by cyclodextrin glycosyltransferase produced by paenibacillus macerans CCM 2012
| Autor: | J. Drahorád, P. Molik, M. Beran, L. Adámek, M. Urban, K. Matušová |
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| Předmět: |
0301 basic medicine
Paenibacillus macerans chemistry.chemical_classification biology Cyclodextrin Chemistry Starch food and beverages Amaranth Cyclodextrin glycosyltransferase 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology Biochemistry Amylopectin biology.protein Amylase Food science Ammonium sulfate precipitation Food Science |
| Zdroj: | Scopus-Elsevier |
| Popis: | Urban M., Beran M., Adamek L., Drahorad J., Molik P., Matusova K. (2012): Cyclodextrin produc - tion from amaranth starch by cyclodextrin glycosyltransferase produced by Paenibacillus macerans rans CCM 2012 was studied in regard to the effect of the starch source (amaranth, maize) on the yield of CDs. CGTase was produced by a 3-day sterile cultivation in the laboratory Bench-top fermentor BiostatB under aerobic conditions. CGTase was partially purified by ammonium sulfate precipitation at 60% saturation. Electrophoretic analysis (SDS-PAGE) of the isolated CGTase enzyme was carried out according to the method by Laemmli (1970), the apparent molecular weight was in the range from 105 kDa to 114 kDa. All the commercially important α-, β-, and γ-CDs were detected chromatographically after the hydrolysis of the maize and amaranth ( amaranthus cruentus ) starches with the isolated enzyme. The amaranth starch appears to be an excellent substrate for CDs production because of the high dispers - ibility, high starch-granule susceptibility to amylases, and the exceptionally high amylopectin content. |
| Databáze: | OpenAIRE |
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