Probiotic Properties and Immunomodulatory Activity of Lactobacillus Strains Isolated from Dairy Products
| Autor: | Silvia Selene Moreno-Guerrero, Arturo Ramírez-Pacheco, Ulises Hernández-Chiñas, Carlos Eslava, Luz M. Rocha-Ramírez |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Lactobacillus casei stress inflammatory 030106 microbiology immunomodulation medicine.disease_cause Microbiology Article law.invention interleukin-8 (IL-8) 03 medical and health sciences Probiotic Lactobacillus rhamnosus law Virology Lactobacillus medicine lcsh:QH301-705.5 nitric oxide (NO) Escherichia coli antimicrobial activity Lactobacillus helveticus biology Chemistry food and beverages Pathogenic bacteria biology.organism_classification 030104 developmental biology lcsh:Biology (General) Lactobacilli Staphylococcus aureus adherence inhibition probiotic |
| Zdroj: | Microorganisms Volume 9 Issue 4 Microorganisms, Vol 9, Iss 825, p 825 (2021) |
| ISSN: | 2076-2607 |
| DOI: | 10.3390/microorganisms9040825 |
| Popis: | Lactobacilli species are an effective biotherapeutic alternative against bacterial infections and intestinal inflammatory disorders. However, it is important to evaluate their beneficial properties, before considering them as probiotics for medical use. In this study we evaluated some probiotic properties of Lactobacillus rhamnosus GG, Lactobacillus rhamnosus KLSD, Lactobacillus helveticus IMAU70129, and Lactobacillus casei IMAU60214 previously isolated from dairy products and as control Lactobacillus casei Shirota. Experimental evaluations revealed that all strains expressed hydrophobicity (25–40%), auto-aggregation (55–60%), NaCl tolerance (1–4%), adhesion to Caco-2 cells (25–33%), partial inhibition on adherence of Escherichia coli ATCC 35218, Salmonella Typhimurium ATCC 14028, and Staphylococcus aureus ATCC 23219. Cell-free supernatants (CFS) of Lactobacilli also inhibit growth of these pathogens. In immunomodulatory properties a reduction of interleukin-8 (IL-8) and nitric oxide (NO) release was observed in assays with Caco-2 cells stimulated with interleukin-1β (1 ng/mL), or lipopolysaccharide (0.1 µg/mL). On the other hand, the damage induced to Caco-2 cells with sodium dodecyl sulfate (SDS) was attenuated when the cultured cells were pretreated with L. rhamnosus KLDS, L. helveticus IMAU70129 and L. casei IMAU60214. These Lactobacilli possess probiotic properties determined by both an antagonistic activity on pathogenic bacteria and reduction in the inflammatory response of cells treated with SDS, a pro-inflammatory stimulant. |
| Databáze: | OpenAIRE |
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