Noninvasive Tests for Diagnosis of Chlamydia trachomatis Infection: Application of Ligase Chain Reaction to First-Catch Urine Specimens of Women
Autor: | John D. Burczak, Howard Shaw, Julius Schachter, Robin Whidden, Jeanne Moncada, Gail Bolan, Hans Lee |
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Rok vydání: | 1995 |
Předmět: |
Chlamydia trachomatis
Cervix Uteri urologic and male genital diseases medicine.disease_cause Sensitivity and Specificity Asymptomatic Urethra Culture Techniques Prevalence medicine Humans Immunology and Allergy Chlamydiaceae Ligase chain reaction Cervix Direct fluorescent antibody Cells Cultured Chlamydia biology Chlamydia Infections medicine.disease biology.organism_classification Virology female genital diseases and pregnancy complications Infectious Diseases medicine.anatomical_structure Fluorescent Antibody Technique Direct Genes Bacterial Chlamydiales Female San Francisco medicine.symptom Nucleic Acid Amplification Techniques human activities Plasmids |
Zdroj: | Journal of Infectious Diseases. 172:1411-1414 |
ISSN: | 1537-6613 0022-1899 |
Popis: | Ligase chain reaction (LCR) to diagnose Chlamydia trachomatis infection was evaluated using first-catch urine (FCU) specimens from 4053 women. Results were compared with those of cell culture (TC) isolation from cervix (all) and urethra (2812 women). The reference standard was TC positivity or positive LCR for chlamydial plasmid DNA confirmed by direct fluorescent antibody test or LCR for another chlamydial gene. Compared with cervical culture, LCR was 88.2% sensitive and 100% specific. Adding urethral culture increased TC sensitivity from 67.1% to 74% and reduced LCR sensitivity to 85.9%. The prevalence of chlamydial infection was 5% (142/2812) by the dual culture system and 5.9% (165/2812) by LCR on FCU specimens. LCR on FCU specimens is highly sensitive and specific for diagnosing chlamydial infection. It is more sensitive than TC and may well present public health authorities with a useful noninvasive screening test for chlamydial infection in asymptomatic women. |
Databáze: | OpenAIRE |
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