Nemo-like kinase (NLK) acts downstream of Notch/Delta signalling to downregulate TCF during mesoderm induction in the sea urchin embryo
| Autor: | Thierry Lepage, Eric Röttinger, Lydia Besnardeau, Christian Gache, Guy Lhomond, Jenifer C. Croce |
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| Přispěvatelé: | Institut de Recherche sur le Cancer et le Vieillissement (IRCAN), Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA), Laboratoire de Biologie du Développement de Villefranche sur mer (LBDV), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut de la Mer de Villefranche (IMEV), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS), Sorbonne Université (SU), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Observatoire océanologique de Villefranche-sur-mer (OOVM), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), Institut de Biologie Valrose (IBV), Université Nice Sophia Antipolis (1965 - 2019) (UNS), Observatoire océanologique de Villefranche-sur-mer (OOVM), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Croce, Jenifer |
| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
MESH: Signal Transduction
MESH: Embryonic Induction MESH: beta Catenin MESH: Amino Acid Sequence Mesoderm 0302 clinical medicine MESH: Gene Expression Regulation Developmental MESH: Animals [SDV.BDD]Life Sciences [q-bio]/Development Biology In Situ Hybridization beta Catenin Genetics Embryonic Induction 0303 health sciences MESH: Mesoderm biology Receptors Notch MESH: Gene Expression Regulation Enzymologic Intracellular Signaling Peptides and Proteins Gene Expression Regulation Developmental Blastula MAP Kinase Kinase Kinases Cell biology medicine.anatomical_structure Phenotype Mitogen-activated protein kinase embryonic structures Paracentrotus MESH: Membrane Proteins Endoderm Mitogen-Activated Protein Kinases TCF Transcription Factors Signal Transduction MESH: Paracentrotus animal structures Molecular Sequence Data Notch signaling pathway MESH: Sequence Alignment Germ layer Lithium MESH: Phenotype Gene Expression Regulation Enzymologic 03 medical and health sciences Endomesoderm MESH: In Situ Hybridization MESH: Intracellular Signaling Peptides and Proteins [SDV.BDD] Life Sciences [q-bio]/Development Biology medicine Animals Amino Acid Sequence Molecular Biology 030304 developmental biology MESH: Molecular Sequence Data MESH: Lithium Membrane Proteins MESH: Mitogen-Activated Protein Kinases Mesoderm formation MESH: MAP Kinase Kinase Kinases biology.protein MESH: Receptors Notch Sequence Alignment 030217 neurology & neurosurgery MESH: TCF Transcription Factors Developmental Biology |
| Zdroj: | Development (Cambridge, England) Development (Cambridge, England), Company of Biologists, 2006, 133 (21), pp.4341-4353. ⟨10.1242/dev.02603⟩ Development (Cambridge, England), 2006, 133 (21), pp.4341-4353. ⟨10.1242/dev.02603⟩ |
| ISSN: | 1477-9129 0950-1991 |
| DOI: | 10.1242/dev.02603⟩ |
| Popis: | International audience; Studies in Caenorhabditis elegans and vertebrates have established that the MAP kinase-related protein NLK counteracts Wnt signalling by downregulating the transcription factor TCF. Here, we present evidence that during early development of the sea urchin embryo, NLK is expressed in the mesodermal precursors in response to Notch signalling and directs their fate by downregulating TCF. The expression pattern of nlk is strikingly similar to that of Delta and the two genes regulate the expression of each other. nlk overexpression, like ectopic activation of Notch signalling, provoked massive formation of mesoderm and associated epithelial mesenchymal transition. NLK function was found to be redundant with that of the MAP kinase ERK during mesoderm formation and to require the activity of the activating kinase TAK1. In addition, the sea urchin NLK, like its vertebrate counterpart, antagonizes the activity of the transcription factor TCF. Finally, activating the expression of a TCF-VP16 construct at blastula stages strongly inhibits endoderm and mesoderm formation, indicating that while TCF activity is required early for launching the endomesoderm gene regulatory network, it has to be downregulated at blastula stage in the mesodermal lineage. Taken together, our results indicate that the evolutionarily conserved TAK/NLK regulatory pathway has been recruited downstream of the Notch/Delta pathway in the sea urchin to switch off TCF-beta-catenin signalling in the mesodermal territory, allowing precursors of this germ layer to segregate from the endomesoderm. |
| Databáze: | OpenAIRE |
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