A multimodal 3D neuro-microphysiological system with neurite-trapping microelectrodes
Autor: | Beatriz Molina-Martínez, Laura-Victoria Jentsch, Fulya Ersoy, Matthijs van der Moolen, Stella Donato, Torbjørn V Ness, Peter Heutink, Peter D Jones, Paolo Cesare |
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Rok vydání: | 2022 |
Předmět: |
Neurons
induced pluripotent stem cells microelectrode array Induced Pluripotent Stem Cells microfluidics Biomedical Engineering Bioengineering General Medicine electrophysiology Biochemistry Electrophysiological Phenomena Biomaterials ddc:570 Neurites Humans microphysiological system 3D engineered neural tissues Microelectrodes Biotechnology |
Zdroj: | Biofabrication 14(2), 025004 (2022). doi:10.1088/1758-5090/ac463b Biofabrication |
ISSN: | 1758-5090 1758-5082 |
Popis: | Three-dimensional cell technologies as pre-clinical models are emerging tools for mimicking the structural and functional complexity of the nervous system. The accurate exploration of phenotypes in engineered 3D neuronal cultures, however, demands morphological, molecular and especially functional measurements. Particularly crucial is measurement of electrical activity of individual neurons with millisecond resolution. Current techniques rely on customized electrophysiological recording set-ups, characterized by limited throughput and poor integration with other readout modalities. Here we describe a novel approach, using multiwell glass microfluidic microelectrode arrays, allowing non-invasive electrical recording from engineered 3D neuronal cultures. We demonstrate parallelized studies with reference compounds, calcium imaging and optogenetic stimulation. Additionally, we show how microplate compatibility allows automated handling and high-content analysis of human induced pluripotent stem cell–derived neurons. This microphysiological platform opens up new avenues for high-throughput studies on the functional, morphological and molecular details of neurological diseases and their potential treatment by therapeutic compounds. |
Databáze: | OpenAIRE |
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