Reactive Oxygen Species-Mediated DNA Damage and Apoptosis in Human Skin Epidermal Cells After Exposure to Nickel Nanoparticles
| Autor: | Saad Alakhtani, Ahmed A. Al-Qahtani, Daoud Ali, Entissar Al Suhaibani, Saud Alarifi |
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| Rok vydání: | 2013 |
| Předmět: |
DNA damage
Endocrinology Diabetes and Metabolism Clinical Biochemistry Metal Nanoparticles Apoptosis medicine.disease_cause Biochemistry Antioxidants Cell Line Inorganic Chemistry Superoxide dismutase Lipid peroxidation chemistry.chemical_compound Microscopy Electron Transmission medicine Humans chemistry.chemical_classification Reactive oxygen species biology Chemistry Biochemistry (medical) General Medicine Molecular biology Acetylcysteine Comet assay Oxidative Stress Catalase biology.protein Reactive Oxygen Species Genotoxicity Oxidative stress DNA Damage |
| Zdroj: | Biological Trace Element Research. 157:84-93 |
| ISSN: | 1559-0720 0163-4984 |
| Popis: | Nickel nanoparticles (NiNPs) are increasingly used in various applications due to their unique properties. However, there is little information concerning the toxicity of NiNPs in the human skin cell (A431). The present study was designed to investigate the cytotoxicity, apoptosis, and DNA damage due to NiNPs in A431 cells. A cellular proliferative capacity test showed that NiNPs induce significant cytotoxicity in a dose- and time-dependent manner. NiNPs were also found to induce oxidative stress evidenced by the generation of reactive oxygen species (ROS) and depletion of glutathione (GSH). Further, co-treatment with the antioxidant N-acetylcysteine (NAC) mitigated the ROS generation due to NiNPs, suggesting the potential mechanism of oxidative stress. NiNPs also induced significant elevation of lipid peroxidation, catalase, and superoxide dismutase and caspase-3 activity in A431 cells. In addition, NAC suppressed NiNP-induced caspase-3 activity. DNA fragmentation analysis using the comet assay showed that the NiNPs cause genotoxicity in a dose- and time-dependent manner. Therefore, the study points out the capability of the NiNPs to induce oxidative stress resulting in apoptosis and genotoxicity. This study warrants more careful assessment of NiNPs before their industrial applications. |
| Databáze: | OpenAIRE |
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