One window-period donation in two years of individual donor-nucleic acid test screening for hepatitis B, hepatitis C and human immunodeficiency virus
| Autor: | M. B. Polite, João Renato Rebello Pinho, Silvia Patricia Nunez, Ricardo Antonio d'Almeida Pereira, José Eduardo Levi, Jose Mauro Kutner, M. Mota |
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| Rok vydání: | 2012 |
| Předmět: |
HBsAg
Hepatitis B virus Hepatitis C virus Window period medicine.disease_cause Blood donors Hepatitis C/diagnosis medicine Reverse transcriptase polymerase chain reaction Hepatitis medicine.diagnostic_test lcsh:RC633-647.5 business.industry Nucleic acid test HIV lcsh:Diseases of the blood and blood-forming organs Hematology Hepatitis C Hepatitis B medicine.disease Virology Immunology Hepatitis B/diagnosis Original Article business |
| Zdroj: | Revista Brasileira de Hematologia e Hemoterapia Revista Brasileira de Hematologia e Hemoterapia v.35 n.3 2013 Revista brasileira de hematologia e hemoterapia Associação Brasileira de Hematologia e Hemoterapia e Terapia Celular (ABHHTC) instacron:ABHHTC Revista Brasileira de Hematologia e Hemoterapia, Volume: 35, Issue: 3, Pages: 167-170, Published: JUN 2013 Revista Brasileira de Hematologia e Hemoterapia, Vol 35, Iss 3, Pp 167-170 (2013) |
| ISSN: | 1516-8484 |
| Popis: | Objective: To describe general data on nucleic acid/serology testing and report the first hepatitis B-nucleic acid testing yield case of an immunized donor in Brazil. Methods: A total of 24,441 donations collected in 2010 and 2011 were submitted to individual nucleic acid testing for hepatitis B, hepatitis C and human immunodeficiency virus using the TaqMan® MPX kit (Roche) on the Cobas s201 platform, in addition to routine screening for serological markers. Nucleic acid testing-reactive donations were further evaluated by real-time polymerase chain reaction using Cobas AmpliPrep/Cobas TaqMan hepatitis B virus, hepatitis C virus and human immunodeficiency virus tests. Results: Thirty-two donations were reactive by nucleic acid testing, 31 were also serologically reactive and one first-time donor was identified as having hepatitis B in the window period. Follow-up samples showed increasing titers of anti-HBs rising from 19 UI/mL in the index donation to 109 IU/mL seven months later attributable to his vaccination history. Curiously, this donor was never reactive for HbsAg nor for anti-HBc. In the yield donation, he was concomitantly reactive for syphilis (enzyme immunoassay and fluorescent treponemal antibody-absorption; venereal disease research laboratory non-reactive). Overall, six donors (0.02%) were characterized as occult hepatitis B. A total of 35% of the confirmed (recombinant immunoblot assay positive) hepatitis C donations were nucleic acid testing non-reactive and no human immunodeficiency virus "elite controller" was identified. Conclusion: The yield rate (1:24,441; 95% confidence interval: 1:9,537 - 1:89,717) contrasts to the North American rate (1:410,540 donations) and strongly advocates the adoption of nucleic acid testing for hepatitis B in Brazil despite the increasing rate of anti-HBs reactive subjects due to the successful immunization program. |
| Databáze: | OpenAIRE |
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