Effect of wounding on chalcone synthase and pathogenesis related PR-10 gene expression and content of phenolic compounds in bilberry leaves

Autor: Laura Jaakola, Kaisu Riihinen, Janne J. Koskimäki, Anja Hohtola, Anne Tolvanen
Rok vydání: 2008
Předmět:
Zdroj: Jaakola, L, Koskimäki, J J, Riihinen, K R, Tolvanen, A & Hohtola, A 2008, ' Effect of wounding on chalcone synthase and pathogenesis related PR-10 gene expression and content of phenolic compounds in bilberry leaves ', Biologia Plantarum, vol. 52, pp. 391-395 . https://doi.org/10.1007/s10535-008-0082-8
ISSN: 1573-8264
0006-3134
Popis: The influence of artificial wounding on biosynthesis of flavonoids and hydroxycinnamic acids was studied in bilberry leaves using two separate wounding experiments. In the first experiment bilberry leaves were wounded by cutting. The expression of the first gene from flavonoid pathway, chalcone synthase (CHS) and a wound induced pathogenesis related PR-10 gene was analysed from samples collected immediately and after 3, 6, 24 h and 4 d from the wounding treatment. In the second experiment annual shoots were removed. Proanthocyanidins, flavonol glycosides and hydroxycinnamic acids were quantified in leaf samples after 0-5 d (experiment 1) and 5 weeks (experiment 2) from the treatment. In the first experiment, no change was observed in the expression of CHS whereas increase in expression of PR-10 gene was detected after 6 h of wounding treatment. In both experiments, the contents of flavonol glycosides and hydroxycinnamic acids were not influenced by the wounding treatment and the contents of proanthocyanidins were decreased.
Databáze: OpenAIRE
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