Serological Diagnosis of Human Herpes Simplex Virus Type 1 and 2 Infections by Luciferase Immunoprecipitation System Assay
| Autor: | Y Hoshino, Tammy Krogmann, Michael J. Iadarola, Ronald L. Hornung, Hannah P. Leahy, Jeffrey I. Cohen, Peter D. Burbelo |
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| Rok vydání: | 2009 |
| Předmět: |
Microbiology (medical)
Herpesvirus 2 Human viruses Molecular Sequence Data Clinical Biochemistry Immunology Herpesvirus 1 Human Biology Antibodies Viral medicine.disease_cause Sensitivity and Specificity Serology Antigen medicine Humans Immunoprecipitation Clinical Laboratory Immunology Immunology and Allergy Serologic Tests Luciferases Antigens Viral medicine.diagnostic_test Antibody titer Herpes Simplex Virology Molecular biology Blot Titer Herpes simplex virus Immunoglobulin G Immunoassay biology.protein Antibody |
| Zdroj: | Clinical and Vaccine Immunology. 16:366-371 |
| ISSN: | 1556-679X 1556-6811 |
| DOI: | 10.1128/cvi.00350-08 |
| Popis: | Highly quantitative and high-throughput serological tests for evaluation of humoral responses to herpes simplex virus 1 (HSV-1) and HSV-2 are not available. The efficacy of luciferase immunoprecipitation system (LIPS) assays for antibody profiling and serologic diagnosis of HSV-1 and HSV-2 infection was investigated using a panel of five recombinant HSV antigens. Plasma samples from subjects seropositive for HSV-1 and/or HSV-2 or seronegative for HSV-1 and HSV-2 that had previously been analyzed by Western blotting and the Focus Plexus immunoassay were evaluated. The LIPS test measuring anti-gG1 antibody titers was 96% sensitive and 96% specific for detecting HSV-1 infection, compared with the Focus immunoassay, and was 92% sensitive and 96% specific, compared with Western blotting. The results for the anti-gG2 LIPS test for HSV-2 precisely matched those for Western blotting, with 100% sensitivity and 100% specificity, and showed robust antibody titers in all the HSV-2-infected samples that were over 1,000 times higher than those in HSV-2-negative or HSV-1-positive samples. Antibodies to three additional HSV-2 proteins, gB, gD, and ICP8, were detected in many of the HSV-1- and/or HSV-2-infected plasma samples and showed preferentially higher immunoreactivity in HSV-2-infected plasma. The titers of antibodies to these three HSV-2 antigens also significantly correlated with each other ( R = 0.75 to 0.81; P < 0.0001). These studies indicate that the robust anti-gG1 and anti-gG2 antibody responses detected by LIPS assays are useful for HSV-1 and HSV-2 detection and suggest that profiling of antibody responses to a panel of HSV proteins may be useful for characterizing individual humoral responses to infection and for monitoring responses to vaccines. |
| Databáze: | OpenAIRE |
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