Engineering of biofilms with a glycosylation circuit for biomaterial applications
| Autor: | Ebru Şahin Kehribar, Musa Efe Isilak, Jozef Adamcik, Raffaele Mezzenga, Urartu Ozgur Safak Seker, Eray Ulas Bozkurt |
|---|---|
| Přispěvatelé: | Şeker, Urartu Özgür Şafak |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Glycosylation
animal structures Biomedical Engineering Biocompatible Materials 02 engineering and technology Bacillus subtilis macromolecular substances medicine.disease_cause Campylobacter jejuni 03 medical and health sciences chemistry.chemical_compound Bacterial Proteins medicine General Materials Science Spider silk Escherichia coli 030304 developmental biology 0303 health sciences biology Biofilm Biomaterial Protein engineering 021001 nanoscience & nanotechnology biology.organism_classification carbohydrates (lipids) Biochemistry chemistry Biofilms lipids (amino acids peptides and proteins) 0210 nano-technology |
| Zdroj: | Biomaterials Science |
| Popis: | Glycosylation is a crucial post-translational modification for a wide range of functionalities. Adhesive protein-based biomaterials in nature rely on heavily glycosylated proteins such as spider silk and mussel adhesive proteins. Engineering protein-based biomaterials genetically enables desired functions and characteristics. Additionally, utilization of glycosylation for biomaterial engineering can expand possibilities by including saccharides to the inventory of building blocks. Here, de novo glycosylation of Bacillus subtilis amyloid-like biofilm protein TasA using a Campylobacter jejuni glycosylation circuit is proposed to be a novel biomaterial engineering method for increasing adhesiveness of TasA fibrils. A C. jejuni glycosylation motif is genetically incorporated to tasA gene and expressed in Escherichia coli containing the C. jejuni pgl protein glycosylation pathway. Glycosylated TasA fibrils indicate enhanced adsorption on the gold surface without disruption of fibril formation. Our findings suggest that N-linked glycosylation can be a promising tool for engineering protein-based biomaterials specifically regarding adhesion. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|