Use of gene targeting for compromising energy homeostasis in neuro-muscular tissues: The role of sarcomeric mitochondrial creatine kinase

Autor: J.M.A. van Deursen, M.L.P. Brückwilder, Arend Heerschap, J.C.M. Koudijs, Benjamin Perryman, Dirk Pette, Karen Steeghs, B. Wieringa, Frank Oerlemans, Paul H.K. Jap, A.F.J. de Haan, Wim Ruitenbeek
Přispěvatelé: Kinesiology
Rok vydání: 1997
Předmět:
Sarcomeres
medicine.medical_specialty
Magnetic Resonance Spectroscopy
Phosphocreatine
Energy metabolism in human skeletal muscle
Cellular respiration
Mutant
Muscle Proteins
Mutagenesis (molecular biology technique)
Oxidative phosphorylation
Mitochondrion
medicine.disease_cause
adaptatie en celdood [Cellulaire herprogrammering in neuromusculaire weefsels in respons op genetische defecten in het cellulaire netwerk voor energie-homeostase]
Oxidative Phosphorylation
Mice
Adenosine Triphosphate
Internal medicine
Intracellulair transport van glycoprotëinen in gepolariseerde epitheelcellen epitheelcellen
medicine
Animals
Homeostasis
NMR of skeletal muscle
Muscle
Skeletal
Creatine Kinase
GeneralLiterature_REFERENCE(e.g.
dictionaries
encyclopedias
glossaries)
Cells
Cultured
Mice
Knockout
Mutation
Adaptation and Cell Death [Cellular reprogramming in tissues of neuro-muscular origin as a response on genetic defects in the network for energy-homeostasis]
biology
General Neuroscience
Gene targeting
Mice
Mutant Strains
Mitochondria
Muscle
Cell biology
Isoenzymes
Endocrinology
Energievoorziening in de menselijke spier onder fysiologische en pathologische condities
Gene Targeting
biology.protein
NMR van skeletspieren
Creatine kinase
Intracellular transport of glycoproteins in polarized epithelial cells
Energy Metabolism
Zdroj: Journal of Neuroscience Methods, 71, 29-41. Elsevier
Journal of Neuroscience Methods, 71, 1, pp. 29-41
Journal of Neuroscience Methods, 71, 29-41
Steeghs, K, Heerschap, A, de Haan, A, Ruitenbeek, W, Oerlemans, F, Deursen, J, Perryman, B, Pette, D, Br|ckwilder, M, Koudijs, J, Jap, P & Wieringa, B 1997, ' Use of gene targeting for compromising energy homeostasis in neuromuscular tissues:The role of sarcomeric mitochondrial creatine kinase ', Journal of Neuroscience Methods, vol. 71, pp. 29-41 . https://doi.org/10.1016/S0165-0270(96)00124-0
Journal of Neuroscience Methods, 71, pp. 29-41
ISSN: 0165-0270
DOI: 10.1016/s0165-0270(96)00124-0
Popis: We have introduced a single knock-out mutation in the mitochondrial creatine kinase gene (ScCKmit) in the mouse germ line via targeted mutagenesis in mouse embryonic stem (ES) cells. Surprisingly, ScCKmit -/- muscles, unlike muscles of mice with a deficiency of cytosolic M-type creatine kinase (M-CK -/-; Van Deursen et al. (1993) Cell 74, 621-631), display no altered morphology, performance or oxidative phosphorylation capacity. Also, the levels of high energy phosphate metabolites were essentially unaltered in ScCKmit mutants. Our results challenge some of the present concepts about the strict coupling between CKmit function and aerobic respiration.
Databáze: OpenAIRE
Externí odkaz: