The plasma membrane Ca2+-ATPase mRNA isoform PMCA 4 is expressed at high levels in neurons of rat piriform cortex and neocortex
| Autor: | Timothy P. Keeton, William L. Stahl, Thomas J. Eakin |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
medicine.medical_specialty
Cerebellum Molecular Sequence Data Hippocampus Calcium-Transporting ATPases Biology Rats Sprague-Dawley Piriform cortex Internal medicine medicine Animals Tissue Distribution RNA Messenger In Situ Hybridization Cerebral Cortex Neurons Neocortex Base Sequence General Neuroscience Cell Membrane Olfactory Pathways Rats Olfactory bulb Cell biology Isoenzymes medicine.anatomical_structure Endocrinology nervous system Cerebral cortex Plasma membrane Ca2+ ATPase Choroid plexus Oligonucleotide Probes |
| Zdroj: | Neuroscience Letters. 178:267-270 |
| ISSN: | 0304-3940 |
| DOI: | 10.1016/0304-3940(94)90775-7 |
| Popis: | Ca2+ transport mediated by the plasma membrane Ca(2+)-ATPase (PMCA) serves an important role in regulation of cytosolic-free Ca2+ in a variety of cells. Isoform PMCA4 mRNA distribution in rat brain was studied by in situ hybridization using 33P-labeled antisense oligodeoxynucleotide probes. Very high levels of hybridization were found in piriform cortex with high levels in amygdaloid nucleus and laminae 2 and 6 of cerebral cortex. Significantly lower levels were found in hypothalamic nuclei and very low or undetectable levels were found in cerebellum, habenula, olfactory bulb, thalamus, choroid plexus of the third and fourth ventricles and in CA1 and CA3 cells of the hippocampus. These results suggest that PMCA4 is not a housekeeping form of the Ca(2+)-ATPase. |
| Databáze: | OpenAIRE |
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