An electrochemiluminescent aptasensor for amplified detection of exosomes from breast tumor cells (MCF-7 cells) based on G-quadruplex/hemin DNAzymes
| Autor: | Bin Qiao, Juqian Jiang, Bangshun He, Qunqun Guo, Jian Shen, Chenxin Cai, Hui Zhang, Yunlong Qi |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Aptamer
Deoxyribozyme Metal Nanoparticles Breast Neoplasms Biosensing Techniques 02 engineering and technology Sulfides Exosomes G-quadruplex 01 natural sciences Biochemistry Analytical Chemistry chemistry.chemical_compound Europium Limit of Detection Cadmium Compounds Electrochemistry Humans Environmental Chemistry 3-Mercaptopropionic Acid Electrodes Spectroscopy Detection limit 010401 analytical chemistry DNA DNA Catalytic Electrochemical Techniques Hydrogen Peroxide Aptamers Nucleotide 021001 nanoscience & nanotechnology Molecular biology Carbon Microvesicles 0104 chemical sciences G-Quadruplexes chemistry MCF-7 Luminescent Measurements MCF-7 Cells Hemin 0210 nano-technology Biomarkers |
| Zdroj: | The Analyst. 144:3668-3675 |
| ISSN: | 1364-5528 0003-2654 |
| Popis: | Exosomes are non-invasive biomarkers for cancer diagnosis. Herein, we describe an electrochemiluminescent (ECL) aptasensor for the detection of exosomes from breast tumor cells. Mercaptopropionic acid (MPA)-modified Eu3+-doped CdS nanocrystals (MPA-CdS:Eu NCs) and H2O2 were used as ECL emitters and coreactant, respectively. The exosomes are recognized and captured by the CD63 aptamer, and then form a G-quadruplex/hemin DNAzyme, which efficiently catalyzes the decomposition of H2O2, resulting in the decreased ECL signal of MPA-CdS:Eu NCs. The exosomes from breast tumor cells (MCF-7 cells) can be detected in the range of 3.4 × 105 to 1.7 × 108 particles per mL. The limit of detection (LOD) was estimated to be 7.41 × 104 particles per mL at a signal-to-noise ratio of 3. The aptasensor has been successfully used to detect exosomes in the serum. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|