Isolation of a novel platform bacterium for lignin valorization and its application in glucose-free cis,cis-muconate production

Autor: Kenji Takahashi, Nanase Abe, Tomonori Sonoki, Naofumi Kamimura, Eri Shinoda, Eiji Masai
Rok vydání: 2019
Předmět:
Zdroj: Journal of Industrial Microbiology and Biotechnology. 46:1071-1080
ISSN: 1476-5535
1367-5435
DOI: 10.1007/s10295-019-02190-6
Popis: Microbial production of cis,cis-muconate (ccMA) from phenolic compounds obtained by chemical depolymerization of lignin is a promising approach to valorize lignin. Because microbial production requires a large amount of carbon and energy source, it is desirable to establish a ccMA-producing strain that utilizes lignin-derived phenols instead of general sources like glucose. We isolated Pseudomonas sp. strain NGC7 that grows well on various phenolic compounds derived from p-hydroxyphenyl, guaiacyl, and syringyl units of lignin. An NGC7 mutant of protocatechuate (PCA) 3,4-dioxygenase and ccMA cycloisomerase genes (NGC703) lost the ability to grow on vanillate and p-hydroxybenzoate but grew normally on syringate. Introduction of a plasmid carrying genes encoding PCA decarboxylase, flavin prenyltransferase, vanillate O-demethylase, and catechol 1,2-dioxygenase into NGC703 enabled production of 3.2 g/L ccMA from vanillate with a yield of 75% while growing on syringate. This strain also produced ccMA from birch lignin-derived phenols. All these results indicate the utility of NGC7 in glucose-free ccMA production.
Databáze: OpenAIRE
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