Determination of aspartate and glutamate in rabbit retina using polymer monolith microextraction coupled to high-performance liquid chromatography with fluorescence detection
| Autor: | Jin-Shu Li, Hong Wang, Yu-Qi Feng, Hui-Juan Zhang |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Monolithic HPLC column
Polymers Glutamic Acid Biochemistry High-performance liquid chromatography Fluorescence Retina Fluorescence spectroscopy Analytical Chemistry Acetic acid chemistry.chemical_compound Animals Sample preparation Monolith Derivatization Chromatography High Pressure Liquid Detection limit Aspartic Acid geography geography.geographical_feature_category Chromatography Molecular Structure Chemistry Hydrogen-Ion Concentration Calibration Hypertension Rabbits |
| Zdroj: | Analytical and Bioanalytical Chemistry. 386:2035-2042 |
| ISSN: | 1618-2650 1618-2642 |
| DOI: | 10.1007/s00216-006-0836-0 |
| Popis: | A simple, sensitive and low-cost method was developed for the determination of aspartate (Asp) and glutamate (Glu) in rabbit retina. Polymer monolith microextraction (PMME) using a poly(acrylamide-vinylpyridine-N,N'-methylene bisacrylamide) (AA-VP-Bis) monolithic column was combined with derivatization of Asp and Glu using 8-phenyl-(4-oxy-acetic acid N-hydroxysuccinimide ester)-4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene (TMPAB-OSu), and this was used to analyze the derivatives of Asp and Glu by high-performance liquid chromatography (HPLC) with fluorescence detection. The conditions for the derivatization and the subsequent extraction of Asp and Glu derivatives were optimized. The enrichment factors for the derivatives of Asp and Glu were found to be 14.1 and 14.7, respectively, by PMME. The limits of detection of Asp and Glu were 0.14 and 0.53 nmol/L, respectively. The precision and recovery were evaluated with spiked retina. The inter- and intraday relative standard deviations were less than 10%. The proposed method was successfully applied to the determination of Asp and Glu levels in rabbit retina samples with different stages of intraocular hypertension. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|