Novel Dehalogenase Mechanism for 2,3-Dichloro-1-Propanol Utilization in Pseudomonas putida Strain MC4
| Autor: | Muhammad I. Arif, Jantien E. Oppentocht, Dick B. Janssen, Ghufrana Samin, Jan G. E. van Leeuwen |
|---|---|
| Přispěvatelé: | Biotechnology |
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
DNA
Bacterial HALOALKANE DEHALOGENASE Magnetic Resonance Spectroscopy Stereochemistry QUINOHEMOPROTEIN ALCOHOL-DEHYDROGENASE Molecular Sequence Data HETEROLOGOUS EXPRESSION ANGSTROM RESOLUTION Dehydrogenase Protein Sorting Signals COMAMONAS-TESTOSTERONI Applied Microbiology and Biotechnology Substrate Specificity chemistry.chemical_compound Organic chemistry Enzyme kinetics Comamonas testosteroni MOLECULAR CHARACTERIZATION Acrolein Cloning Molecular HALOALCOHOL DEHALOGENASE Dehalogenase Ecology biology Sequence Homology Amino Acid Pseudomonas putida Sequence Analysis DNA biology.organism_classification DEPENDENT ALDEHYDE DEHYDROGENASE Carbon Kinetics XANTHOBACTER-AUTOTROPHICUS GJ10 1-Propanol chemistry QUINOPROTEIN ETHANOL DEHYDROGENASE Biodegradation Energy source Energy Metabolism Oxidoreductases Chlorohydrins Food Science Biotechnology Haloalkane dehalogenase |
| Zdroj: | Applied and environmental microbiology, 78(17), 6128-6136. AMER SOC MICROBIOLOGY |
| ISSN: | 1098-5336 0099-2240 |
| Popis: | A Pseudomonas putida strain (MC4) that can utilize 2,3-dichloro-1-propanol (DCP) and several aliphatic haloacids and haloalcohols as sole carbon and energy source for growth was isolated from contaminated soil. Degradation of DCP was found to start with oxidation and concomitant dehalogenation catalyzed by a 72-kDa monomeric protein (DppA) that was isolated from cell lysate. The dppA gene was cloned from a cosmid library and appeared to encode a protein equipped with a signal peptide and that possessed high similarity to quinohemoprotein alcohol dehydrogenases (ADHs), particularly ADH IIB and ADH IIG from Pseudomonas putida HK. This novel dehalogenating dehydrogenase has a broad substrate range, encompassing a number of nonhalogenated alcohols and haloalcohols. With DCP, DppA exhibited a k cat of 17 s −1 . 1 H nuclear magnetic resonance experiments indicated that DCP oxidation by DppA in the presence of 2,6-dichlorophenolindophenol (DCPIP) and potassium ferricyanide [K 3 Fe(CN) 6 ] yielded 2-chloroacrolein, which was oxidized to 2-chloroacrylic acid. |
| Databáze: | OpenAIRE |
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